The DIANA-microT web server v5.0 is an updated version of the microRNA target prediction and functional analysis tool, which has been significantly enhanced with an improved target prediction algorithm, DIANA-microT-CDS. This new version incorporates miRBase version 18 and Ensembl version 69, and supports miRNA-gene interactions in Homo sapiens, Mus musculus, Drosophila melanogaster, and Caenorhabditis elegans. The web server has been redesigned to host sophisticated workflows that can be used directly from the online interface, enabling users without advanced bioinformatics infrastructure to perform advanced multi-step functional miRNA analyses. Key features include support for high-throughput data analysis, integration with the Taverna Workflow Management System (WMS) using the DIANA-Taverna Plug-in, and the availability of ready-made advanced workflows for miRNA-related analyses. The server also provides programmatic access to multiple DIANA algorithms and supports advanced result filtering based on tissue expression and uploaded gene lists. Overall, the new version aims to enhance the accuracy and usability of miRNA target prediction and functional analysis, facilitating complex miRNA-related analyses for researchers.The DIANA-microT web server v5.0 is an updated version of the microRNA target prediction and functional analysis tool, which has been significantly enhanced with an improved target prediction algorithm, DIANA-microT-CDS. This new version incorporates miRBase version 18 and Ensembl version 69, and supports miRNA-gene interactions in Homo sapiens, Mus musculus, Drosophila melanogaster, and Caenorhabditis elegans. The web server has been redesigned to host sophisticated workflows that can be used directly from the online interface, enabling users without advanced bioinformatics infrastructure to perform advanced multi-step functional miRNA analyses. Key features include support for high-throughput data analysis, integration with the Taverna Workflow Management System (WMS) using the DIANA-Taverna Plug-in, and the availability of ready-made advanced workflows for miRNA-related analyses. The server also provides programmatic access to multiple DIANA algorithms and supports advanced result filtering based on tissue expression and uploaded gene lists. Overall, the new version aims to enhance the accuracy and usability of miRNA target prediction and functional analysis, facilitating complex miRNA-related analyses for researchers.