This study presents a DNA-based self-assembly method to create chiral plasmonic nanostructures with tailored optical responses. By using DNA origami, the researchers arranged gold nanoparticles into nanoscale helices with high spatial accuracy. These helical structures exhibit giant circular dichroism (CD) and optical rotary dispersion (ORD) in the visible range, arising from collective plasmon interactions. The optical response can be tuned by altering the composition of the metal nanoparticles. The chiral response is independent of the incident light's propagation direction and can be switched between left- and right-handed orientations. The method allows for the precise spatial arrangement of nanoparticles, enabling the fabrication of complex materials with tailored optical properties. The study demonstrates the potential of DNA self-assembly for creating plasmonic nanostructures with applications in negative refractive index materials, cloaking, and perfect lenses. The approach is scalable and can be used to create materials with isotropic optical activity. The results show that the optical activity of the self-assembled nanohelices can be significantly enhanced by increasing the size of the nanoparticles or by using alloy shells. The study also highlights the potential of DNA-based self-assembly for the design and fabrication of optically active materials with customized spectral responses. The method is demonstrated through experiments showing the optical activity of the self-assembled nanohelices, including the optical rotary dispersion effect. The results indicate the potential of DNA-based self-assembly for creating materials with unique optical properties. The study provides a comprehensive understanding of the optical properties of chiral plasmonic nanostructures and their potential applications in various fields.This study presents a DNA-based self-assembly method to create chiral plasmonic nanostructures with tailored optical responses. By using DNA origami, the researchers arranged gold nanoparticles into nanoscale helices with high spatial accuracy. These helical structures exhibit giant circular dichroism (CD) and optical rotary dispersion (ORD) in the visible range, arising from collective plasmon interactions. The optical response can be tuned by altering the composition of the metal nanoparticles. The chiral response is independent of the incident light's propagation direction and can be switched between left- and right-handed orientations. The method allows for the precise spatial arrangement of nanoparticles, enabling the fabrication of complex materials with tailored optical properties. The study demonstrates the potential of DNA self-assembly for creating plasmonic nanostructures with applications in negative refractive index materials, cloaking, and perfect lenses. The approach is scalable and can be used to create materials with isotropic optical activity. The results show that the optical activity of the self-assembled nanohelices can be significantly enhanced by increasing the size of the nanoparticles or by using alloy shells. The study also highlights the potential of DNA-based self-assembly for the design and fabrication of optically active materials with customized spectral responses. The method is demonstrated through experiments showing the optical activity of the self-assembled nanohelices, including the optical rotary dispersion effect. The results indicate the potential of DNA-based self-assembly for creating materials with unique optical properties. The study provides a comprehensive understanding of the optical properties of chiral plasmonic nanostructures and their potential applications in various fields.