This study introduces a non-genetically engineered artificial mechanoreceptor (AMR) that can reprogram non-mechanoresponsive receptor tyrosine kinases (RTKs) to sense user-defined force cues, enabling *de novo* designed mechanotransduction. The AMR is a modular DNA-protein chimera comprising a mechanosensing-and-transmitting DNA nanodevice grafted on natural RTKs via aptameric anchors. The DNA mechano-switch senses intercellular tensile forces, actuating a force-triggered dynamic DNA assembly to manipulate RTK dimerization and activate intracellular signaling. The versatility of AMR allows the reprogramming of c-Met and FGFR1 RTKs to customize mechanobiological functions, such as adhesion-mediated neural stem cell maintenance. The AMR platform offers predictable and tunable force sensitivity, versatility for cellular force inputs, dynamic visualization of actuation, and plug-and-play installation for diverse output receptor signaling. This work provides a valuable toolkit for understanding mechanobiology and developing force-responsible cell-based therapies.This study introduces a non-genetically engineered artificial mechanoreceptor (AMR) that can reprogram non-mechanoresponsive receptor tyrosine kinases (RTKs) to sense user-defined force cues, enabling *de novo* designed mechanotransduction. The AMR is a modular DNA-protein chimera comprising a mechanosensing-and-transmitting DNA nanodevice grafted on natural RTKs via aptameric anchors. The DNA mechano-switch senses intercellular tensile forces, actuating a force-triggered dynamic DNA assembly to manipulate RTK dimerization and activate intracellular signaling. The versatility of AMR allows the reprogramming of c-Met and FGFR1 RTKs to customize mechanobiological functions, such as adhesion-mediated neural stem cell maintenance. The AMR platform offers predictable and tunable force sensitivity, versatility for cellular force inputs, dynamic visualization of actuation, and plug-and-play installation for diverse output receptor signaling. This work provides a valuable toolkit for understanding mechanobiology and developing force-responsible cell-based therapies.