DNA barcoding is a novel technique used to identify and classify organisms, particularly plants. This review provides an overview of the progress in DNA barcoding within plant taxonomy, highlighting the success of various barcode loci, the emergence of super barcodes from the chloroplast genome, and the impact of next-generation sequencing technologies. The method involves amplifying, sequencing, and comparing DNA barcodes with reference databases to achieve accurate species identification. While the mitochondrial gene *COI* is widely used in animals, no universal DNA barcode exists for plants. The review discusses the challenges and limitations of using single-locus barcodes and proposes the use of combinations of multiple loci, such as matK and rbcL, to improve species discrimination. The development of super barcodes, which utilize the entire chloroplast genome, offers higher resolution for distinguishing closely related species. Additionally, specific barcodes and mini barcoding methods are explored to address the limitations of traditional approaches. The future of plant DNA barcoding lies in leveraging next-generation sequencing technologies, particularly in generating super barcodes, to enhance the precision of species identification and biodiversity assessments.DNA barcoding is a novel technique used to identify and classify organisms, particularly plants. This review provides an overview of the progress in DNA barcoding within plant taxonomy, highlighting the success of various barcode loci, the emergence of super barcodes from the chloroplast genome, and the impact of next-generation sequencing technologies. The method involves amplifying, sequencing, and comparing DNA barcodes with reference databases to achieve accurate species identification. While the mitochondrial gene *COI* is widely used in animals, no universal DNA barcode exists for plants. The review discusses the challenges and limitations of using single-locus barcodes and proposes the use of combinations of multiple loci, such as matK and rbcL, to improve species discrimination. The development of super barcodes, which utilize the entire chloroplast genome, offers higher resolution for distinguishing closely related species. Additionally, specific barcodes and mini barcoding methods are explored to address the limitations of traditional approaches. The future of plant DNA barcoding lies in leveraging next-generation sequencing technologies, particularly in generating super barcodes, to enhance the precision of species identification and biodiversity assessments.