The paper describes a new method for determining nucleotide sequences in DNA using chain-terminating inhibitors. This method, similar to the "plus and minus" technique, employs 2′,3′-dideoxy and arabinonucleoside analogues of deoxynucleoside triphosphates, which act as specific inhibitors of DNA polymerase. The technique is applied to the DNA of bacteriophage φX174 and is more rapid and accurate than the "plus and minus" method. The principle involves incorporating these inhibitors into the growing DNA chain, causing termination at specific residues. The method is demonstrated through experiments using different inhibitors and restriction enzyme fragments, showing that it can determine sequences with reasonable accuracy. The results are clearer and more reliable compared to other methods, but the availability of all necessary inhibitors, particularly ddGTP, remains a challenge. The authors emphasize the importance of confirming sequence results using additional techniques or priming on the opposite strand.The paper describes a new method for determining nucleotide sequences in DNA using chain-terminating inhibitors. This method, similar to the "plus and minus" technique, employs 2′,3′-dideoxy and arabinonucleoside analogues of deoxynucleoside triphosphates, which act as specific inhibitors of DNA polymerase. The technique is applied to the DNA of bacteriophage φX174 and is more rapid and accurate than the "plus and minus" method. The principle involves incorporating these inhibitors into the growing DNA chain, causing termination at specific residues. The method is demonstrated through experiments using different inhibitors and restriction enzyme fragments, showing that it can determine sequences with reasonable accuracy. The results are clearer and more reliable compared to other methods, but the availability of all necessary inhibitors, particularly ddGTP, remains a challenge. The authors emphasize the importance of confirming sequence results using additional techniques or priming on the opposite strand.