ENDscript 2 is an advanced web server designed for extracting and rendering comprehensive protein structure information, from primary to quaternary levels, in an automated manner. This major upgrade enhances speed, accuracy, and usability with interactive 3D visualization. It leverages the new version 3 of ESPript, a well-known sequence alignment renderer, to handle large datasets efficiently. ENDscript 2 can generate high-quality figures displaying multiple sequence alignments of homologous proteins, colored according to residue conservation, along with experimental secondary structure elements and detailed biophysical and structural data. These elements are mapped onto interactive 3D PyMOL representations, allowing users to fine-tune settings to their needs. The server also supports the visualization of multiple biochemical and structural data from external biotool web servers, both in 2D and 3D formats. ENDscript 2 is freely available at http://endscript.ibcp.fr and http://esprirt.ibcp.fr, respectively. The article includes a case study on the crystal structure of Proteus mirabilis catalase (PDB entry 2CAH) to demonstrate the tool's capabilities.ENDscript 2 is an advanced web server designed for extracting and rendering comprehensive protein structure information, from primary to quaternary levels, in an automated manner. This major upgrade enhances speed, accuracy, and usability with interactive 3D visualization. It leverages the new version 3 of ESPript, a well-known sequence alignment renderer, to handle large datasets efficiently. ENDscript 2 can generate high-quality figures displaying multiple sequence alignments of homologous proteins, colored according to residue conservation, along with experimental secondary structure elements and detailed biophysical and structural data. These elements are mapped onto interactive 3D PyMOL representations, allowing users to fine-tune settings to their needs. The server also supports the visualization of multiple biochemical and structural data from external biotool web servers, both in 2D and 3D formats. ENDscript 2 is freely available at http://endscript.ibcp.fr and http://esprirt.ibcp.fr, respectively. The article includes a case study on the crystal structure of Proteus mirabilis catalase (PDB entry 2CAH) to demonstrate the tool's capabilities.