ENDscript 2 is a Web server that automatically extracts and displays comprehensive information about protein structures, from primary to quaternary. It uses the new version 3 of ESPript, which is faster and more efficient, and provides interactive 3D visualizations. The server can process large datasets and generate high-quality figures with multiple sequence alignments, colored according to residue conservation. It also displays experimental secondary structure elements and biophysical data. ENDscript 2 is an open platform for visualizing multiple biochemical and structural data from external sources. It allows users to customize settings and generate interactive 3D representations using PyMOL. The server also provides a downloadable MSA file in Stockholm format and hypertext links to PDB records. ENDscript 2 has been improved with new features, including support for 12 genome databases, enhanced MSA programs, and a more user-friendly interface. It is designed to be fast and convenient, with an automated pipeline and parallel processing. ENDscript 2 is a valuable tool for biologists and structural biologists, enabling the generation of detailed high-quality figures and interactive 3D representations of proteins. It is also an effective educational tool for teaching structural biology. The server is freely available at http://endscript.ibcp.fr and http://espript.ibcp.fr.ENDscript 2 is a Web server that automatically extracts and displays comprehensive information about protein structures, from primary to quaternary. It uses the new version 3 of ESPript, which is faster and more efficient, and provides interactive 3D visualizations. The server can process large datasets and generate high-quality figures with multiple sequence alignments, colored according to residue conservation. It also displays experimental secondary structure elements and biophysical data. ENDscript 2 is an open platform for visualizing multiple biochemical and structural data from external sources. It allows users to customize settings and generate interactive 3D representations using PyMOL. The server also provides a downloadable MSA file in Stockholm format and hypertext links to PDB records. ENDscript 2 has been improved with new features, including support for 12 genome databases, enhanced MSA programs, and a more user-friendly interface. It is designed to be fast and convenient, with an automated pipeline and parallel processing. ENDscript 2 is a valuable tool for biologists and structural biologists, enabling the generation of detailed high-quality figures and interactive 3D representations of proteins. It is also an effective educational tool for teaching structural biology. The server is freely available at http://endscript.ibcp.fr and http://espript.ibcp.fr.