Deep eutectic solvents (DESs) are emerging as eco-friendly alternatives to traditional solvents like ethanol and n-hexane. They are non-toxic, biodegradable, and water-miscible with low volatility. DESs are mainly synthesized through heating and stirring, and their physicochemical properties such as polarity, viscosity, density, and surface tension influence their applications. This review discusses the use of DESs in the extraction of bioactive compounds from various agricultural products, including fruits, vegetables, cereals, pulses, spices, herbs, plantation crops, oil seed crops, medicinal and aromatic plants, seaweed, and milk. DESs are also used for the determination of pesticides, insecticides, heavy metals, illegal milk additives, and the purification of antibiotics. The review also covers the safety regulations of DESs and their potential applications in cosmetics and food industry. DESs have various applications in food science, such as the extraction of undesired compounds, improving mechanical properties of food packaging films, acting as anti-freezing agents, encapsulating low bioavailable compounds, and enhancing organoleptic properties. DESs are also used in other areas like membrane preparation for ultrafiltration and nanofiltration, and in the separation of azeotropic mixtures. The preparation methods of DESs include heating and stirring, microwave, ultrasonication, grinding, freeze drying, and vacuum evaporation. The physicochemical properties of DESs, such as density, viscosity, polarity, and surface tension, are influenced by factors like temperature, molar ratio of HBA and HBD, and pressure. DESs have shown promising results in the extraction of bioactive compounds from various agricultural commodities and their by-products, with applications in food, cosmetics, and pharmaceuticals. The review highlights the potential of DESs in the food industry and their role in sustainable and eco-friendly processes.Deep eutectic solvents (DESs) are emerging as eco-friendly alternatives to traditional solvents like ethanol and n-hexane. They are non-toxic, biodegradable, and water-miscible with low volatility. DESs are mainly synthesized through heating and stirring, and their physicochemical properties such as polarity, viscosity, density, and surface tension influence their applications. This review discusses the use of DESs in the extraction of bioactive compounds from various agricultural products, including fruits, vegetables, cereals, pulses, spices, herbs, plantation crops, oil seed crops, medicinal and aromatic plants, seaweed, and milk. DESs are also used for the determination of pesticides, insecticides, heavy metals, illegal milk additives, and the purification of antibiotics. The review also covers the safety regulations of DESs and their potential applications in cosmetics and food industry. DESs have various applications in food science, such as the extraction of undesired compounds, improving mechanical properties of food packaging films, acting as anti-freezing agents, encapsulating low bioavailable compounds, and enhancing organoleptic properties. DESs are also used in other areas like membrane preparation for ultrafiltration and nanofiltration, and in the separation of azeotropic mixtures. The preparation methods of DESs include heating and stirring, microwave, ultrasonication, grinding, freeze drying, and vacuum evaporation. The physicochemical properties of DESs, such as density, viscosity, polarity, and surface tension, are influenced by factors like temperature, molar ratio of HBA and HBD, and pressure. DESs have shown promising results in the extraction of bioactive compounds from various agricultural commodities and their by-products, with applications in food, cosmetics, and pharmaceuticals. The review highlights the potential of DESs in the food industry and their role in sustainable and eco-friendly processes.