Deep Eutectic Solvents (DESs) are innovative, eco-benign solvents that have gained attention for their non-toxic, biodegradable, and water-miscible properties. This review article provides an overview of the preparation methods, physicochemical properties, and applications of DESs in various agricultural commodities and their by-products. DESs are synthesized through methods such as heating and stirring, microwave, ultrasonication, grinding, freeze-drying, and vacuum evaporation. Their physicochemical properties, including density, viscosity, polarity, and surface tension, influence their applications. DESs are used for the extraction of bioactive compounds from fruits, vegetables, cereals, pulses, spices, herbs, plantation crops, oil seed crops, medicinal and aromatic plants, seaweed, and milk. They are also employed in the determination of pesticides, insecticides, hazardous compounds, heavy metals, illegal milk additives, and the purification of antibiotics. Additionally, DESs are used in the preparation of packaging films and membrane preparation for ultrafiltration and nanofiltration. The review highlights the advantages of DESs over traditional solvents and discusses safety regulations and potential applications in the food industry.Deep Eutectic Solvents (DESs) are innovative, eco-benign solvents that have gained attention for their non-toxic, biodegradable, and water-miscible properties. This review article provides an overview of the preparation methods, physicochemical properties, and applications of DESs in various agricultural commodities and their by-products. DESs are synthesized through methods such as heating and stirring, microwave, ultrasonication, grinding, freeze-drying, and vacuum evaporation. Their physicochemical properties, including density, viscosity, polarity, and surface tension, influence their applications. DESs are used for the extraction of bioactive compounds from fruits, vegetables, cereals, pulses, spices, herbs, plantation crops, oil seed crops, medicinal and aromatic plants, seaweed, and milk. They are also employed in the determination of pesticides, insecticides, hazardous compounds, heavy metals, illegal milk additives, and the purification of antibiotics. Additionally, DESs are used in the preparation of packaging films and membrane preparation for ultrafiltration and nanofiltration. The review highlights the advantages of DESs over traditional solvents and discusses safety regulations and potential applications in the food industry.