The study demonstrates the successful expression and application of the CRISPR/Cas9/sgRNA system in targeted gene modification in four plant species: Arabidopsis, tobacco, rice, and sorghum. The researchers used *Agrobacterium tumefaciens* to deliver the Cas9 gene and sgRNA to the plants, targeting specific DNA sequences in the GFP gene and the promoter regions of the bacterial blight susceptibility genes *OsSWEET14* and *OsSWEET11* in rice. The results showed that the Cas9/sgRNA complex successfully cleaved the target DNA, leading to mutagenesis and restoration of gene function in Arabidopsis and tobacco, and mutation in rice. The study provides evidence that the Cas9/sgRNA system is a powerful tool for plant genetic engineering, with potential applications in both scientific research and agriculture.The study demonstrates the successful expression and application of the CRISPR/Cas9/sgRNA system in targeted gene modification in four plant species: Arabidopsis, tobacco, rice, and sorghum. The researchers used *Agrobacterium tumefaciens* to deliver the Cas9 gene and sgRNA to the plants, targeting specific DNA sequences in the GFP gene and the promoter regions of the bacterial blight susceptibility genes *OsSWEET14* and *OsSWEET11* in rice. The results showed that the Cas9/sgRNA complex successfully cleaved the target DNA, leading to mutagenesis and restoration of gene function in Arabidopsis and tobacco, and mutation in rice. The study provides evidence that the Cas9/sgRNA system is a powerful tool for plant genetic engineering, with potential applications in both scientific research and agriculture.