This review article explores the development and applications of fibrin-based biomaterials and scaffolds in tissue engineering. Fibrin, a naturally occurring protein, is highlighted for its unique properties, including high biocompatibility, non-toxicity, and degradability. The article discusses the composition, structure, and biological properties of fibrin, emphasizing its role in hemostasis, anti-inflammation, and wound healing. It also covers the modification and cross-linking methods of fibrin, such as methacryloyl reaction, sulfonation, and chemical cross-linking, which enhance its mechanical strength and stability. The review further details the diverse applications of fibrin, including fibrin hydrogels, fibrin glue, and fibrin microbeads, each with specific advantages for tissue and organ repair. Fibrin hydrogels are noted for their porous structure and high porosity, promoting cell migration and angiogenesis. Fibrin glue, or fibrin sealant, is effective in intraoperative hemostasis and tissue adhesion. Fibrin microbeads, composed of dense spherical beads, provide an ECM-like environment for cell migration and proliferation, aiding in tissue regeneration. The article concludes by discussing future research directions in this field, emphasizing the potential of fibrin-based biomaterials in advancing tissue engineering and clinical treatments.This review article explores the development and applications of fibrin-based biomaterials and scaffolds in tissue engineering. Fibrin, a naturally occurring protein, is highlighted for its unique properties, including high biocompatibility, non-toxicity, and degradability. The article discusses the composition, structure, and biological properties of fibrin, emphasizing its role in hemostasis, anti-inflammation, and wound healing. It also covers the modification and cross-linking methods of fibrin, such as methacryloyl reaction, sulfonation, and chemical cross-linking, which enhance its mechanical strength and stability. The review further details the diverse applications of fibrin, including fibrin hydrogels, fibrin glue, and fibrin microbeads, each with specific advantages for tissue and organ repair. Fibrin hydrogels are noted for their porous structure and high porosity, promoting cell migration and angiogenesis. Fibrin glue, or fibrin sealant, is effective in intraoperative hemostasis and tissue adhesion. Fibrin microbeads, composed of dense spherical beads, provide an ECM-like environment for cell migration and proliferation, aiding in tissue regeneration. The article concludes by discussing future research directions in this field, emphasizing the potential of fibrin-based biomaterials in advancing tissue engineering and clinical treatments.