Differences in the Localization and Morphology of Chromosomes in the Human Nucleus

Differences in the Localization and Morphology of Chromosomes in the Human Nucleus

June 14, 1999 | Jenny A. Croft, Joanna M. Bridger, Shelagh Boyle, Paul Perry, Peter Teague, and Wendy A. Bickmore
This study investigates the nuclear localization and morphology of human chromosomes 18 and 19 using fluorescence in situ hybridization (FISH). The results show that chromosome 18 is more peripheral in the nucleus compared to chromosome 19, which is more centrally located. These differences are maintained throughout the cell cycle and are reflected in the orientation of translocation chromosomes. Inhibition of transcription and histone deacetylation affects chromosome morphology but not their nuclear position. The study also demonstrates that the distribution of genomic sequences between chromosomes influences nuclear structure, suggesting that specific regions of chromosomes may have distinct nuclear fates. The findings highlight the importance of chromosomal positioning and its implications for gene regulation and evolutionary constraints.This study investigates the nuclear localization and morphology of human chromosomes 18 and 19 using fluorescence in situ hybridization (FISH). The results show that chromosome 18 is more peripheral in the nucleus compared to chromosome 19, which is more centrally located. These differences are maintained throughout the cell cycle and are reflected in the orientation of translocation chromosomes. Inhibition of transcription and histone deacetylation affects chromosome morphology but not their nuclear position. The study also demonstrates that the distribution of genomic sequences between chromosomes influences nuclear structure, suggesting that specific regions of chromosomes may have distinct nuclear fates. The findings highlight the importance of chromosomal positioning and its implications for gene regulation and evolutionary constraints.
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