The study investigates the nature of information conveyed by a single neuron to multiple target neurons, focusing on neocortical pyramidal neurons. Triple and quadruple neuron recordings revealed that each synaptic connection established by these neurons is potentially unique, differing in the number and dendritic locations of synaptic contacts, absolute synaptic strengths, rates of synaptic depression and recovery. The same axon of a pyramidal neuron can mediate frequency-dependent depression and facilitation in different target neurons, suggesting that the nature of the target neurons underlies qualitative differences in synaptic properties. Facilitating-type synaptic connections from three pyramidal neurons to a single interneuron showed similar qualitative features but quantitative differences, indicating that different pre-post synaptic interactions underlie these differences. Mathematical analysis of the transfer functions of frequency-dependent synapses revealed supralinear, linear, and sub-linear signaling regimes, allowing multiple synaptic representations of the same presynaptic action potential train. The heterogeneity in synaptic transfer functions suggests that differential signaling is a key mechanism in neocortical information processing, regulated by selective synaptic modifications.The study investigates the nature of information conveyed by a single neuron to multiple target neurons, focusing on neocortical pyramidal neurons. Triple and quadruple neuron recordings revealed that each synaptic connection established by these neurons is potentially unique, differing in the number and dendritic locations of synaptic contacts, absolute synaptic strengths, rates of synaptic depression and recovery. The same axon of a pyramidal neuron can mediate frequency-dependent depression and facilitation in different target neurons, suggesting that the nature of the target neurons underlies qualitative differences in synaptic properties. Facilitating-type synaptic connections from three pyramidal neurons to a single interneuron showed similar qualitative features but quantitative differences, indicating that different pre-post synaptic interactions underlie these differences. Mathematical analysis of the transfer functions of frequency-dependent synapses revealed supralinear, linear, and sub-linear signaling regimes, allowing multiple synaptic representations of the same presynaptic action potential train. The heterogeneity in synaptic transfer functions suggests that differential signaling is a key mechanism in neocortical information processing, regulated by selective synaptic modifications.