This review discusses the latest advancements in diagnostic and management strategies for plant viral diseases, focusing on RNA-based approaches such as RNA interference (RNAi) and double-stranded RNA (dsRNA). It highlights the potential of these molecular tools to revolutionize agriculture by reducing reliance on pesticides, minimizing crop damage, and enhancing sustainability. The review explores the role of non-coding RNAs (ncRNAs) in controlling viral infections and emphasizes the importance of precise dsRNA design for effective viral RNA targeting while minimizing harm to plant RNA. Despite challenges such as off-target effects and efficient dsRNA delivery, RNA-based strategies are seen as transformative in agriculture. The review also compares RNAi methods with CRISPR-Cas technologies and discusses the application of RNA-aided CRISPR-Cas gene-editing methods to prevent plant viral infections. It addresses the recent documentation of new plant viruses impacting vegetable production in the USA and covers the two primary methods for engineering virus resistance. The review emphasizes the challenges that must be addressed before these technologies can be widely employed to protect crops from viruses. It also discusses various detection methods, including serological techniques, PCR-based thermal amplification, isothermal amplification assays, and big data methods such as microarrays and high-throughput sequencing. These methods are crucial for the rapid and accurate diagnosis of plant viral diseases. The review highlights the importance of these strategies in preventing aphid-mediated plant virus transmission and underscores the need for continued research and development to improve the effectiveness and applicability of these technologies in agriculture.This review discusses the latest advancements in diagnostic and management strategies for plant viral diseases, focusing on RNA-based approaches such as RNA interference (RNAi) and double-stranded RNA (dsRNA). It highlights the potential of these molecular tools to revolutionize agriculture by reducing reliance on pesticides, minimizing crop damage, and enhancing sustainability. The review explores the role of non-coding RNAs (ncRNAs) in controlling viral infections and emphasizes the importance of precise dsRNA design for effective viral RNA targeting while minimizing harm to plant RNA. Despite challenges such as off-target effects and efficient dsRNA delivery, RNA-based strategies are seen as transformative in agriculture. The review also compares RNAi methods with CRISPR-Cas technologies and discusses the application of RNA-aided CRISPR-Cas gene-editing methods to prevent plant viral infections. It addresses the recent documentation of new plant viruses impacting vegetable production in the USA and covers the two primary methods for engineering virus resistance. The review emphasizes the challenges that must be addressed before these technologies can be widely employed to protect crops from viruses. It also discusses various detection methods, including serological techniques, PCR-based thermal amplification, isothermal amplification assays, and big data methods such as microarrays and high-throughput sequencing. These methods are crucial for the rapid and accurate diagnosis of plant viral diseases. The review highlights the importance of these strategies in preventing aphid-mediated plant virus transmission and underscores the need for continued research and development to improve the effectiveness and applicability of these technologies in agriculture.