DnaSP v5 is a comprehensive software package for analyzing DNA polymorphism data, designed to handle large datasets and multiple data files. It includes new features such as haplotype phasing, analysis of insertion/deletion polymorphisms (DIPs), and visualization of sliding window results integrated with genome annotations in the UCSC browser. The software supports various DNA sequence alignment formats and offers a user-friendly graphical interface for data management and analysis. Key improvements include the ability to handle batch processing of multiple data files, statistical methods for haplotype reconstruction, and the estimation of DIP summary statistics. DnaSP v5 is freely available to academic users and runs on Windows, Apple Macintosh, Linux, and Unix platforms.DnaSP v5 is a comprehensive software package for analyzing DNA polymorphism data, designed to handle large datasets and multiple data files. It includes new features such as haplotype phasing, analysis of insertion/deletion polymorphisms (DIPs), and visualization of sliding window results integrated with genome annotations in the UCSC browser. The software supports various DNA sequence alignment formats and offers a user-friendly graphical interface for data management and analysis. Key improvements include the ability to handle batch processing of multiple data files, statistical methods for haplotype reconstruction, and the estimation of DIP summary statistics. DnaSP v5 is freely available to academic users and runs on Windows, Apple Macintosh, Linux, and Unix platforms.