This study focuses on the isolation and characterization of a lead-resistant bacterium, *Enterobacter kobei* FACU6, from industrial wastewater in Egypt. The bacterium demonstrated an efficient lead removal rate of 83.4% and a remarkable lead absorption capacity of 571.9 mg/g dry weight, with a maximum tolerance concentration (MTC) of 3,000 mg/L. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) revealed significant lead adsorption and intracellular accumulation in treated bacteria. Whole-genome sequencing of *E. kobei* FACU6 revealed a genome size of 4,856,454 bp with a G + C content of 55.06%. The genome contains 4,655 coding sequences (CDS), 75 rRNA genes, and 4 rRNA genes, and includes genes associated with heavy metal resistance and regulatory elements. Gene expression analysis under specific environmental conditions, including pH 7, temperature of 30°C, and high concentrations of heavy metals, showed statistically significant upregulation of four specific heavy metal resistance genes. The findings highlight the potential of *E. kobei* FACU6 as a valuable candidate for developing safe and effective strategies for heavy metal disposal due to its diverse genes related to heavy metal resistance and plant growth promotion.This study focuses on the isolation and characterization of a lead-resistant bacterium, *Enterobacter kobei* FACU6, from industrial wastewater in Egypt. The bacterium demonstrated an efficient lead removal rate of 83.4% and a remarkable lead absorption capacity of 571.9 mg/g dry weight, with a maximum tolerance concentration (MTC) of 3,000 mg/L. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) revealed significant lead adsorption and intracellular accumulation in treated bacteria. Whole-genome sequencing of *E. kobei* FACU6 revealed a genome size of 4,856,454 bp with a G + C content of 55.06%. The genome contains 4,655 coding sequences (CDS), 75 rRNA genes, and 4 rRNA genes, and includes genes associated with heavy metal resistance and regulatory elements. Gene expression analysis under specific environmental conditions, including pH 7, temperature of 30°C, and high concentrations of heavy metals, showed statistically significant upregulation of four specific heavy metal resistance genes. The findings highlight the potential of *E. kobei* FACU6 as a valuable candidate for developing safe and effective strategies for heavy metal disposal due to its diverse genes related to heavy metal resistance and plant growth promotion.