This study presents a draft genome analysis of Enterobacter kobei FACU6, a promising lead-resistant bacterium with potential for bioremediation. The bacterium was isolated from industrial wastewater in Egypt and demonstrated high lead resistance, with a lead removal rate of 83.4% and a lead absorption capacity of 571.9 mg/g dry weight. It also exhibited a maximum tolerance concentration (MTC) of 3,000 mg/L for lead. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) revealed significant lead adsorption and intracellular accumulation in the treated bacteria. Whole-genome sequencing revealed a genome size of 4,856,454 bp with a G + C content of 55.06%, encoding 4,655 coding sequences (CDS), 75 tRNA genes, and 4 rRNA genes. The genome contains genes associated with heavy metal resistance and their corresponding regulatory elements. The expression levels of four specific heavy metal resistance genes were evaluated, showing statistically significant upregulation under specific environmental conditions. The genome also contains genes related to plant growth promotion, making E. kobei FACU6 a valuable candidate for developing safe and effective strategies for heavy metal disposal. The study highlights the potential of E. kobei FACU6 as a source of diverse genes related to heavy metal resistance and plant growth promotion. The findings suggest that E. kobei FACU6 is a multi-heavy metal resistant isolate with high tolerance concentrations for Pb, As, Cr, and Cd. The genome analysis provides insights into the genetic mechanisms underlying its lead resistance and its potential for bioremediation applications. The study also demonstrates the importance of genome analysis in understanding the genetic basis of heavy metal resistance and its potential applications in environmental remediation.This study presents a draft genome analysis of Enterobacter kobei FACU6, a promising lead-resistant bacterium with potential for bioremediation. The bacterium was isolated from industrial wastewater in Egypt and demonstrated high lead resistance, with a lead removal rate of 83.4% and a lead absorption capacity of 571.9 mg/g dry weight. It also exhibited a maximum tolerance concentration (MTC) of 3,000 mg/L for lead. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) revealed significant lead adsorption and intracellular accumulation in the treated bacteria. Whole-genome sequencing revealed a genome size of 4,856,454 bp with a G + C content of 55.06%, encoding 4,655 coding sequences (CDS), 75 tRNA genes, and 4 rRNA genes. The genome contains genes associated with heavy metal resistance and their corresponding regulatory elements. The expression levels of four specific heavy metal resistance genes were evaluated, showing statistically significant upregulation under specific environmental conditions. The genome also contains genes related to plant growth promotion, making E. kobei FACU6 a valuable candidate for developing safe and effective strategies for heavy metal disposal. The study highlights the potential of E. kobei FACU6 as a source of diverse genes related to heavy metal resistance and plant growth promotion. The findings suggest that E. kobei FACU6 is a multi-heavy metal resistant isolate with high tolerance concentrations for Pb, As, Cr, and Cd. The genome analysis provides insights into the genetic mechanisms underlying its lead resistance and its potential for bioremediation applications. The study also demonstrates the importance of genome analysis in understanding the genetic basis of heavy metal resistance and its potential applications in environmental remediation.