A study conducted in the Valle del Cauca department, Colombia, evaluated the seroconversion response to rabies vaccination in dogs. The study involved 569 dogs vaccinated in 42 municipalities. Sera were collected and analyzed using quantitative ELISA to measure rabies-neutralizing antibodies. Results showed that 9.1% of dogs were seronegative, and 25.1% did not show an adequate humoral immune response. Antibody levels decreased over time, correlating with age and vaccine quality but not with sex or breed. The study recommended using viable vaccines, administering two doses in the first six months of life, and annual booster shots. It also emphasized the need for health authorities to monitor vaccination programs. The study highlighted the importance of vaccination in maintaining immunity and reducing rabies transmission. Key findings included lower antibody levels in older dogs and those vaccinated by private entities. The study also identified factors contributing to poor immune responses, such as low-quality vaccines and lack of annual boosters. Overall, the study underscores the need for improved vaccination practices to ensure effective immunity against rabies.A study conducted in the Valle del Cauca department, Colombia, evaluated the seroconversion response to rabies vaccination in dogs. The study involved 569 dogs vaccinated in 42 municipalities. Sera were collected and analyzed using quantitative ELISA to measure rabies-neutralizing antibodies. Results showed that 9.1% of dogs were seronegative, and 25.1% did not show an adequate humoral immune response. Antibody levels decreased over time, correlating with age and vaccine quality but not with sex or breed. The study recommended using viable vaccines, administering two doses in the first six months of life, and annual booster shots. It also emphasized the need for health authorities to monitor vaccination programs. The study highlighted the importance of vaccination in maintaining immunity and reducing rabies transmission. Key findings included lower antibody levels in older dogs and those vaccinated by private entities. The study also identified factors contributing to poor immune responses, such as low-quality vaccines and lack of annual boosters. Overall, the study underscores the need for improved vaccination practices to ensure effective immunity against rabies.