ESHRE guideline: Management of women with premature ovarian insufficiency (POI) provides best practice advice for the care of women with POI, both primary and secondary. The guideline was developed by a multidisciplinary group of experts using the ESHRE guideline development methodology, including systematic literature searches, quality assessment of included papers up to September 2014, and consensus within the guideline group. The guideline includes 99 recommendations addressing 31 key questions on the diagnosis and treatment of POI. It covers diagnosis, assessment, management, and the consequences of POI on fertility, bone health, cardiovascular health, psychological wellbeing, and neurological function. The guideline also addresses the implications for relatives of women with POI, and provides recommendations on hormone replacement therapy, alternative treatments, and puberty induction. The guideline is limited by the lack of data, with many recommendations based on expert opinion or indirect evidence from studies on postmenopausal women or women with Turner syndrome. Despite these limitations, the guideline group is confident that this document will guide healthcare professionals in providing the best practice for managing women with POI based on current evidence. The guideline is intended for update every four years, with an intermediate assessment two years after publication. The guideline is available for review at http://mc.manuscriptcentral.com/humrep.ESHRE guideline: Management of women with premature ovarian insufficiency (POI) provides best practice advice for the care of women with POI, both primary and secondary. The guideline was developed by a multidisciplinary group of experts using the ESHRE guideline development methodology, including systematic literature searches, quality assessment of included papers up to September 2014, and consensus within the guideline group. The guideline includes 99 recommendations addressing 31 key questions on the diagnosis and treatment of POI. It covers diagnosis, assessment, management, and the consequences of POI on fertility, bone health, cardiovascular health, psychological wellbeing, and neurological function. The guideline also addresses the implications for relatives of women with POI, and provides recommendations on hormone replacement therapy, alternative treatments, and puberty induction. The guideline is limited by the lack of data, with many recommendations based on expert opinion or indirect evidence from studies on postmenopausal women or women with Turner syndrome. Despite these limitations, the guideline group is confident that this document will guide healthcare professionals in providing the best practice for managing women with POI based on current evidence. The guideline is intended for update every four years, with an intermediate assessment two years after publication. The guideline is available for review at http://mc.manuscriptcentral.com/humrep.