ESPr ipt is a program designed to visualize multiple sequence alignments in PostScript format, facilitating the analysis and publication of sequence similarities and secondary structure information. It can read pre-aligned sequences and secondary structure files, such as those generated by CLUSTAL-W or DSSP, and produce colorful PostScript files that highlight important regions in the alignment. The program offers a command-line interface and an HTML-based user interface, allowing users to sort and highlight residues based on conservation and secondary structure. ESPr ipt can also integrate information from multiple secondary structure files and generate PDB files with similarity scores. The program is written in FORTRAN77 and is available for various platforms, with a CGI script for web-based execution. It has been developed and refined at several institutions in France and the UK, and is accessible via its website and anonymous FTP.ESPr ipt is a program designed to visualize multiple sequence alignments in PostScript format, facilitating the analysis and publication of sequence similarities and secondary structure information. It can read pre-aligned sequences and secondary structure files, such as those generated by CLUSTAL-W or DSSP, and produce colorful PostScript files that highlight important regions in the alignment. The program offers a command-line interface and an HTML-based user interface, allowing users to sort and highlight residues based on conservation and secondary structure. ESPr ipt can also integrate information from multiple secondary structure files and generate PDB files with similarity scores. The program is written in FORTRAN77 and is available for various platforms, with a CGI script for web-based execution. It has been developed and refined at several institutions in France and the UK, and is accessible via its website and anonymous FTP.