**Summary:** This study evaluates the impact of payment for environmental services (PES) through an eco-efficiency index in cattle farms in the La Vieja River basin, Quindío, and Valle del Cauca, Colombia. The research aims to assess the effectiveness of livestock conversion through ecosystem service (ES) indicators at various scales. ES indicators of carbon storage and biodiversity conservation were analyzed for 104 farms participating in a PES project. A sample of 20 farms was selected based on typologies identified through cluster analysis, and synthetic ES indices related to soil quality, landscape changes, and a socio-economic indicator were constructed and evaluated using multivariate statistical procedures. The soil index evaluated in 2003 showed improved ES quality in silvopastoral systems (SSPs). The landscape index showed a high correlation with biodiversity and carbon ES. Finally, an approach to the concept of eco-efficiency was made by integrating the different indices and indicators, finding better performance in farms with higher ES values of carbon, biodiversity, and landscape. Key terms: environmental service indicators, ecosystem services, sustainable livestock, silvopastoral systems, eco-efficiency. The study highlights the importance of eco-efficiency in assessing the impact of PES on livestock farms, emphasizing the need for sustainable practices to conserve biodiversity and carbon storage. The research contributes to understanding the role of SSPs in promoting environmental sustainability and the effectiveness of PES in improving ecosystem services in agricultural systems. The findings suggest that integrating eco-efficiency indices can provide a comprehensive assessment of the environmental impact of livestock farming and support the development of sustainable agricultural practices.**Summary:** This study evaluates the impact of payment for environmental services (PES) through an eco-efficiency index in cattle farms in the La Vieja River basin, Quindío, and Valle del Cauca, Colombia. The research aims to assess the effectiveness of livestock conversion through ecosystem service (ES) indicators at various scales. ES indicators of carbon storage and biodiversity conservation were analyzed for 104 farms participating in a PES project. A sample of 20 farms was selected based on typologies identified through cluster analysis, and synthetic ES indices related to soil quality, landscape changes, and a socio-economic indicator were constructed and evaluated using multivariate statistical procedures. The soil index evaluated in 2003 showed improved ES quality in silvopastoral systems (SSPs). The landscape index showed a high correlation with biodiversity and carbon ES. Finally, an approach to the concept of eco-efficiency was made by integrating the different indices and indicators, finding better performance in farms with higher ES values of carbon, biodiversity, and landscape. Key terms: environmental service indicators, ecosystem services, sustainable livestock, silvopastoral systems, eco-efficiency. The study highlights the importance of eco-efficiency in assessing the impact of PES on livestock farms, emphasizing the need for sustainable practices to conserve biodiversity and carbon storage. The research contributes to understanding the role of SSPs in promoting environmental sustainability and the effectiveness of PES in improving ecosystem services in agricultural systems. The findings suggest that integrating eco-efficiency indices can provide a comprehensive assessment of the environmental impact of livestock farming and support the development of sustainable agricultural practices.