This study investigates the effect of 4-META/MMA-TBB resin on the regeneration and cell attachment of oral epithelium following gingivectomy in rats. The resin, a widely used dental adhesive, was applied to the entire wound surface after gingival surgery, and the regenerating epithelium was examined at various time points. The results show that the regenerating epithelium under the resin did not undergo keratinization but became keratinized immediately after the resin was removed. Immunohistochemical analysis revealed that laminin 5 and integrin β4 were localized in the basal lamina and at the resin-regenerating epithelium interface, while cytokeratin 14 was expressed in the regenerating epithelium underneath the resin and in healthy and regenerated junctional epithelial cells. These findings suggest that the resin covers the wound surface and facilitates the biological adherence of the regenerating epithelium during its initial regeneration process.This study investigates the effect of 4-META/MMA-TBB resin on the regeneration and cell attachment of oral epithelium following gingivectomy in rats. The resin, a widely used dental adhesive, was applied to the entire wound surface after gingival surgery, and the regenerating epithelium was examined at various time points. The results show that the regenerating epithelium under the resin did not undergo keratinization but became keratinized immediately after the resin was removed. Immunohistochemical analysis revealed that laminin 5 and integrin β4 were localized in the basal lamina and at the resin-regenerating epithelium interface, while cytokeratin 14 was expressed in the regenerating epithelium underneath the resin and in healthy and regenerated junctional epithelial cells. These findings suggest that the resin covers the wound surface and facilitates the biological adherence of the regenerating epithelium during its initial regeneration process.