This study investigates the effect of 4-META/MMA-TBB resin on the adhesion and keratinization of regenerating oral epithelium following gingivectomy. The resin, commonly used as a dental adhesive and wound dressing, was applied to the wound surface in rats and its impact on epithelial regeneration was evaluated. The study examined the expression of laminin 5, integrin β4, and cytokeratin 14 in the regenerating epithelium. Results showed that epithelium under the resin was not keratinized but became keratinized after resin removal. Laminin 5 and integrin β4 were localized in the basal lamina, internal basal lamina, and at the resin-epithelium interface. Cytokeratin 14 was found in the regenerating epithelium and healthy junctional epithelium. These findings suggest that the resin covers the wound surface and the regenerating epithelium biologically adheres to the resin during the initial stages of regeneration. The study also found that the resin application did not affect the healing process or the rate of regeneration, but inhibited keratinization. After resin removal, the epithelium showed signs of keratinization similar to healthy tissue. The results indicate that the resin provides a supportive environment for epithelial regeneration, but once removed, the epithelium regains its normal characteristics. The study highlights the role of laminin 5 and integrin β4 in epithelial adhesion and the importance of these proteins in the regeneration process.This study investigates the effect of 4-META/MMA-TBB resin on the adhesion and keratinization of regenerating oral epithelium following gingivectomy. The resin, commonly used as a dental adhesive and wound dressing, was applied to the wound surface in rats and its impact on epithelial regeneration was evaluated. The study examined the expression of laminin 5, integrin β4, and cytokeratin 14 in the regenerating epithelium. Results showed that epithelium under the resin was not keratinized but became keratinized after resin removal. Laminin 5 and integrin β4 were localized in the basal lamina, internal basal lamina, and at the resin-epithelium interface. Cytokeratin 14 was found in the regenerating epithelium and healthy junctional epithelium. These findings suggest that the resin covers the wound surface and the regenerating epithelium biologically adheres to the resin during the initial stages of regeneration. The study also found that the resin application did not affect the healing process or the rate of regeneration, but inhibited keratinization. After resin removal, the epithelium showed signs of keratinization similar to healthy tissue. The results indicate that the resin provides a supportive environment for epithelial regeneration, but once removed, the epithelium regains its normal characteristics. The study highlights the role of laminin 5 and integrin β4 in epithelial adhesion and the importance of these proteins in the regeneration process.