This study investigates the effects of incorporating *Enterococcus faecium* and *Streptococcus thermophilus* (DSP) using a novel premix-spray method on various aspects of broiler chickens, including growth rate, digestive enzyme activity, antioxidant levels, gut microbiome composition, and morphological characteristics of the duodenum, jejunum, and ileum. The study also explores the potential benefits of DSP in reducing flatulence, regulating stool microbial population, and improving diarrhea symptoms. **Key Findings:** - **Growth Rate:** DSP significantly improved specific growth rate (SGR), survival rate (SR), and body weight gain (BWG) while decreasing the feed conversion ratio (FCR). - **Digestive Enzyme Activity:** DSP enhanced the activity of digestive enzymes (protease, amylase, lipase, and trypsin) and increased antioxidant potential (SOD, MDA, and catalase). - **Gut Microbiome:** DSP treatments significantly increased microvillus height in the duodenum and jejunum but had no notable effects in the ileum. DSP also improved the relative abundance of *Ruminococcaceae* and *Faecalibacterium*, leading to better management of diarrhea and reduced presence of *E. coli*. - **Morphological Characteristics:** DSP treatments resulted in significant enhancements in the tissue morphology of the duodenum and jejunum. - **Gas Emissions:** DSP supplementation reduced H₂S, CO₂, NH₃, and CH₄ levels in manure. **Conclusion:** The study suggests that the new spray-drying approach with these strains has potential for supplementing probiotics in poultry feed processing, and including DSP in broiler chicken diets has beneficial effects on growth, digestion, and gut health.This study investigates the effects of incorporating *Enterococcus faecium* and *Streptococcus thermophilus* (DSP) using a novel premix-spray method on various aspects of broiler chickens, including growth rate, digestive enzyme activity, antioxidant levels, gut microbiome composition, and morphological characteristics of the duodenum, jejunum, and ileum. The study also explores the potential benefits of DSP in reducing flatulence, regulating stool microbial population, and improving diarrhea symptoms. **Key Findings:** - **Growth Rate:** DSP significantly improved specific growth rate (SGR), survival rate (SR), and body weight gain (BWG) while decreasing the feed conversion ratio (FCR). - **Digestive Enzyme Activity:** DSP enhanced the activity of digestive enzymes (protease, amylase, lipase, and trypsin) and increased antioxidant potential (SOD, MDA, and catalase). - **Gut Microbiome:** DSP treatments significantly increased microvillus height in the duodenum and jejunum but had no notable effects in the ileum. DSP also improved the relative abundance of *Ruminococcaceae* and *Faecalibacterium*, leading to better management of diarrhea and reduced presence of *E. coli*. - **Morphological Characteristics:** DSP treatments resulted in significant enhancements in the tissue morphology of the duodenum and jejunum. - **Gas Emissions:** DSP supplementation reduced H₂S, CO₂, NH₃, and CH₄ levels in manure. **Conclusion:** The study suggests that the new spray-drying approach with these strains has potential for supplementing probiotics in poultry feed processing, and including DSP in broiler chicken diets has beneficial effects on growth, digestion, and gut health.