The study investigates the use of Starburst polyamidoamine (PAMAM) dendrimers as a novel method for efficient genetic material transfer into mammalian cells. PAMAM dendrimers, known for their unique structural and physical properties, were synthesized and characterized using various analytical techniques. The efficiency of plasmid DNA transfection using these dendrimers was evaluated using two reporter gene systems: firefly luciferase and bacterial β-galactosidase. The results showed that highly efficient transfection of a broad range of eukaryotic cells and cell lines was achieved with minimal cytotoxicity. However, the transfection efficiency varied depending on the type of dendrimer and the presence of additional compounds, such as DEAE-dextran, which altered the nature of the complex. Some cell lines demonstrated enhanced transfection with the addition of chloroquine, indicating endosomal localization of the complexes. The ability of a dendrimer to transfect cells appeared to depend on its size, shape, and the number of primary amino groups on its surface. The study concludes that Starburst dendrimers can transfect a wide variety of cell types in vitro and offer an efficient method for producing permanently transfected cell lines, with potential applications in both in vitro and in vivo gene transfer.The study investigates the use of Starburst polyamidoamine (PAMAM) dendrimers as a novel method for efficient genetic material transfer into mammalian cells. PAMAM dendrimers, known for their unique structural and physical properties, were synthesized and characterized using various analytical techniques. The efficiency of plasmid DNA transfection using these dendrimers was evaluated using two reporter gene systems: firefly luciferase and bacterial β-galactosidase. The results showed that highly efficient transfection of a broad range of eukaryotic cells and cell lines was achieved with minimal cytotoxicity. However, the transfection efficiency varied depending on the type of dendrimer and the presence of additional compounds, such as DEAE-dextran, which altered the nature of the complex. Some cell lines demonstrated enhanced transfection with the addition of chloroquine, indicating endosomal localization of the complexes. The ability of a dendrimer to transfect cells appeared to depend on its size, shape, and the number of primary amino groups on its surface. The study concludes that Starburst dendrimers can transfect a wide variety of cell types in vitro and offer an efficient method for producing permanently transfected cell lines, with potential applications in both in vitro and in vivo gene transfer.