This paper presents a novel method to enhance the antimicrobial action of plasma-activated hydrogel therapy (PAHT) for wound decontamination. The process involves grounding and hydrating poly(vinyl alcohol) (PVA) hydrogel films during treatment with a helium (He) plasma jet, which electrochemically enhances the production of hydrogen peroxide (H₂O₂), a major antibacterial agent. The production of H₂O₂ is facilitated through electron dissociation reactions and reactions involving excited state species, metastables, and UV photolysis. Additionally, the He flow dehydrates the PVA hydrogel, chemically enhancing H₂O₂ production. The electrochemically enhanced PAHT produces an unprecedented 3.4 mM of H₂O₂ in the PVA hydrogel, which is highly effective against common wound pathogens such as *Escherichia coli* and *Pseudomonas aeruginosa*, and mildly effective against *Staphylococcus aureus*. The study demonstrates that this new PAHT dressing offers a promising alternative to antibiotics and silver-based dressings for controlling infection and stimulating healing in wounds.This paper presents a novel method to enhance the antimicrobial action of plasma-activated hydrogel therapy (PAHT) for wound decontamination. The process involves grounding and hydrating poly(vinyl alcohol) (PVA) hydrogel films during treatment with a helium (He) plasma jet, which electrochemically enhances the production of hydrogen peroxide (H₂O₂), a major antibacterial agent. The production of H₂O₂ is facilitated through electron dissociation reactions and reactions involving excited state species, metastables, and UV photolysis. Additionally, the He flow dehydrates the PVA hydrogel, chemically enhancing H₂O₂ production. The electrochemically enhanced PAHT produces an unprecedented 3.4 mM of H₂O₂ in the PVA hydrogel, which is highly effective against common wound pathogens such as *Escherichia coli* and *Pseudomonas aeruginosa*, and mildly effective against *Staphylococcus aureus*. The study demonstrates that this new PAHT dressing offers a promising alternative to antibiotics and silver-based dressings for controlling infection and stimulating healing in wounds.