This paper presents an electrochemical method to enhance the antimicrobial action of plasma-activated hydrogel therapy (PAHT) in wound decontamination. The method involves grounding and hydrating poly(vinyl alcohol) (PVA) hydrogel films during treatment with a helium (He) plasma jet. This electrochemical process enhances the production of hydrogen peroxide (H₂O₂), a major antibacterial agent, through electron dissociation reactions and reactions involving excited state species, metastables, and UV photolysis. The He flow of the plasma jet dehydrates the PVA hydrogel, fueling electrochemical reactions that increase H₂O₂ production. The process produces an unprecedented 3.4 mM of H₂O₂ in the PVA hydrogel. Other molecules like reactive nitrogen species (RNS) are also enhanced by this method. The electrochemically enhanced PAHT is highly effective against common wound pathogens such as Escherichia coli and Pseudomonas aeruginosa, and mildly effective against Staphylococcus aureus. The study shows that the new PAHT dressing offers a promising alternative to antibiotics and silver-based dressings for controlling infection and stimulating healing in wounds. The PVA hydrogel is chosen for its regulatory approval, mechanical and biocompatibility properties, and high ionic conductivity, which promotes electrochemical reactions. The hydrogel is activated by the He plasma jet in a humidified atmosphere, enhancing H₂O₂ production through electron dissociation reactions and UV photolysis. The study also demonstrates that grounding the PVA hydrogel during plasma treatment significantly enhances H₂O₂ and NO₂⁻ production, which are important for decontaminating and healing wounds. The results show that the PVA hydrogel can release high concentrations of H₂O₂ and NO₂⁻, which are effective against bacterial pathogens. The study also shows that the PVA hydrogel can maintain its antibacterial activity even after storage in a conventional freezer for several days. The results indicate that the electrochemical enhancement of PAHT significantly improves its antimicrobial effectiveness and potential for wound treatment.This paper presents an electrochemical method to enhance the antimicrobial action of plasma-activated hydrogel therapy (PAHT) in wound decontamination. The method involves grounding and hydrating poly(vinyl alcohol) (PVA) hydrogel films during treatment with a helium (He) plasma jet. This electrochemical process enhances the production of hydrogen peroxide (H₂O₂), a major antibacterial agent, through electron dissociation reactions and reactions involving excited state species, metastables, and UV photolysis. The He flow of the plasma jet dehydrates the PVA hydrogel, fueling electrochemical reactions that increase H₂O₂ production. The process produces an unprecedented 3.4 mM of H₂O₂ in the PVA hydrogel. Other molecules like reactive nitrogen species (RNS) are also enhanced by this method. The electrochemically enhanced PAHT is highly effective against common wound pathogens such as Escherichia coli and Pseudomonas aeruginosa, and mildly effective against Staphylococcus aureus. The study shows that the new PAHT dressing offers a promising alternative to antibiotics and silver-based dressings for controlling infection and stimulating healing in wounds. The PVA hydrogel is chosen for its regulatory approval, mechanical and biocompatibility properties, and high ionic conductivity, which promotes electrochemical reactions. The hydrogel is activated by the He plasma jet in a humidified atmosphere, enhancing H₂O₂ production through electron dissociation reactions and UV photolysis. The study also demonstrates that grounding the PVA hydrogel during plasma treatment significantly enhances H₂O₂ and NO₂⁻ production, which are important for decontaminating and healing wounds. The results show that the PVA hydrogel can release high concentrations of H₂O₂ and NO₂⁻, which are effective against bacterial pathogens. The study also shows that the PVA hydrogel can maintain its antibacterial activity even after storage in a conventional freezer for several days. The results indicate that the electrochemical enhancement of PAHT significantly improves its antimicrobial effectiveness and potential for wound treatment.