Electrochemiluminescence (ECL) microscopy is an emerging technique that leverages the unique properties of ECL to provide high-resolution imaging of biological entities. This review highlights recent advances in ECL imaging technology, emphasizing innovative configurations that enable the imaging of biological entities and enhance analytical properties through increased complexity and multiplexing of bioassays. ECL imaging can be performed in two main modes: ECL⁺, where ECL-emitting objects are viewed as bright against a dark background, and ECL⁻, where insulating objects are imaged as dark against a bright background. These modes provide valuable information about the analyzed system, allowing for the study of biological processes and the observation of discrete events in solution. Recent developments include super-resolution ECL microscopy, which achieves sub-diffraction limit imaging, and the imaging of single molecules and events, such as single photons and biomolecules. ECL microscopy has also been applied to single-cell analysis, revealing detailed morphological and dynamic changes in living cells. Additionally, ECL has been used to image subcellular structures and specific biomolecules on tissue sections, offering insights into cellular biology and clinical diagnostics. The review concludes by discussing the challenges and future directions for ECL microscopy, including the need for brighter emitters, more versatile luminophores, and the integration of ECL into other scientific fields.Electrochemiluminescence (ECL) microscopy is an emerging technique that leverages the unique properties of ECL to provide high-resolution imaging of biological entities. This review highlights recent advances in ECL imaging technology, emphasizing innovative configurations that enable the imaging of biological entities and enhance analytical properties through increased complexity and multiplexing of bioassays. ECL imaging can be performed in two main modes: ECL⁺, where ECL-emitting objects are viewed as bright against a dark background, and ECL⁻, where insulating objects are imaged as dark against a bright background. These modes provide valuable information about the analyzed system, allowing for the study of biological processes and the observation of discrete events in solution. Recent developments include super-resolution ECL microscopy, which achieves sub-diffraction limit imaging, and the imaging of single molecules and events, such as single photons and biomolecules. ECL microscopy has also been applied to single-cell analysis, revealing detailed morphological and dynamic changes in living cells. Additionally, ECL has been used to image subcellular structures and specific biomolecules on tissue sections, offering insights into cellular biology and clinical diagnostics. The review concludes by discussing the challenges and future directions for ECL microscopy, including the need for brighter emitters, more versatile luminophores, and the integration of ECL into other scientific fields.