This study presents a proof-of-concept for a novel implantable fibrous membrane designed to treat periodontitis. The membrane is fabricated using multiple-fluid electrospinning, resulting in a trilayer eccentric side-by-side structure. The outermost layer contains a hydrophilic polymer and ketoprofen, providing rapid pain relief and anti-inflammatory effects. The middle layer is loaded with metronidazole for sustained release, while the innermost layer is loaded with nano-hydroxyapatite to promote alveolar bone growth. Experimental results show that the implant films have good wettability, mechanical properties, biodegradability, and antibacterial properties. They reduce inflammatory responses, promote osteoblast formation, and enhance fibroblast attachment, infiltration, proliferation, and differentiation. Overall, the developed fibrous implant films show strong potential for combined treatment of periodontitis, offering a new approach to creating multi-chamber advanced fiber materials with robust process-structure-performance relationships.This study presents a proof-of-concept for a novel implantable fibrous membrane designed to treat periodontitis. The membrane is fabricated using multiple-fluid electrospinning, resulting in a trilayer eccentric side-by-side structure. The outermost layer contains a hydrophilic polymer and ketoprofen, providing rapid pain relief and anti-inflammatory effects. The middle layer is loaded with metronidazole for sustained release, while the innermost layer is loaded with nano-hydroxyapatite to promote alveolar bone growth. Experimental results show that the implant films have good wettability, mechanical properties, biodegradability, and antibacterial properties. They reduce inflammatory responses, promote osteoblast formation, and enhance fibroblast attachment, infiltration, proliferation, and differentiation. Overall, the developed fibrous implant films show strong potential for combined treatment of periodontitis, offering a new approach to creating multi-chamber advanced fiber materials with robust process-structure-performance relationships.