Bilosomes, derived from bile acids, are advanced nanocarriers for drug delivery, offering enhanced stability and permeability. This review highlights their potential in various therapeutic areas, including brain disorders, ocular diseases, oral vaccines, protein/peptide delivery, and cancer treatment. Bilosomes improve drug bioavailability by protecting therapeutic agents from degradation in the gastrointestinal tract and enhancing mucosal penetration. Their amphiphilic nature, aided by bile salts, enhances drug solubility and permeability, making them effective for both hydrophilic and lipophilic compounds. In brain disorders, bilosomes enable targeted delivery of drugs like resveratrol and zolmitriptan, improving therapeutic outcomes. For ocular delivery, bilosomes enhance transcorneal flux and bioavailability, as demonstrated in studies on antifungal and anti-inflammatory agents. In oral vaccine delivery, bilosomes provide a stable and effective means of antigen delivery, improving immune responses. For proteins and peptides, bilosomes enhance stability and permeability, overcoming challenges in oral administration. In cancer treatment, bilosomes improve drug delivery to tumor cells, as shown in studies on anticancer agents. Overall, bilosomes show promise as a versatile nanocarrier, offering improved efficacy and safety in various drug delivery applications.Bilosomes, derived from bile acids, are advanced nanocarriers for drug delivery, offering enhanced stability and permeability. This review highlights their potential in various therapeutic areas, including brain disorders, ocular diseases, oral vaccines, protein/peptide delivery, and cancer treatment. Bilosomes improve drug bioavailability by protecting therapeutic agents from degradation in the gastrointestinal tract and enhancing mucosal penetration. Their amphiphilic nature, aided by bile salts, enhances drug solubility and permeability, making them effective for both hydrophilic and lipophilic compounds. In brain disorders, bilosomes enable targeted delivery of drugs like resveratrol and zolmitriptan, improving therapeutic outcomes. For ocular delivery, bilosomes enhance transcorneal flux and bioavailability, as demonstrated in studies on antifungal and anti-inflammatory agents. In oral vaccine delivery, bilosomes provide a stable and effective means of antigen delivery, improving immune responses. For proteins and peptides, bilosomes enhance stability and permeability, overcoming challenges in oral administration. In cancer treatment, bilosomes improve drug delivery to tumor cells, as shown in studies on anticancer agents. Overall, bilosomes show promise as a versatile nanocarrier, offering improved efficacy and safety in various drug delivery applications.