Engineering the Caenorhabditis elegans genome using Cas9-triggered homologous recombination

Engineering the Caenorhabditis elegans genome using Cas9-triggered homologous recombination

2013-10-01 | Daniel J Dickinson, Jordan D Ward, David J Reiner, Bob Goldstein
This article describes a method for genome editing in *Caenorhabditis elegans* using Cas9-triggered homologous recombination. The authors demonstrate that Cas9 can induce double-strand breaks at specific sites in the *C. elegans* genome and that these breaks can be repaired efficiently by homologous recombination. By supplying engineered homologous repair templates, they generated *gfp* knock-ins and targeted mutations. The method is flexible, cost-effective, and robust, allowing for the rapid and precise modification of the *C. elegans* genome. The authors also discuss the advantages of this approach over other methods, such as Mos1-mediated transposon excision and conventional transgenic techniques, and address potential limitations and off-target effects. Overall, the study highlights the potential of Cas9-triggered homologous recombination for a wide range of genetic experiments in *C. elegans*.This article describes a method for genome editing in *Caenorhabditis elegans* using Cas9-triggered homologous recombination. The authors demonstrate that Cas9 can induce double-strand breaks at specific sites in the *C. elegans* genome and that these breaks can be repaired efficiently by homologous recombination. By supplying engineered homologous repair templates, they generated *gfp* knock-ins and targeted mutations. The method is flexible, cost-effective, and robust, allowing for the rapid and precise modification of the *C. elegans* genome. The authors also discuss the advantages of this approach over other methods, such as Mos1-mediated transposon excision and conventional transgenic techniques, and address potential limitations and off-target effects. Overall, the study highlights the potential of Cas9-triggered homologous recombination for a wide range of genetic experiments in *C. elegans*.
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