The paper introduces an innovative strategy for point-of-care testing (POCT) using a 3D-printed hollow microneedle array patch (MAP) and a vacuum tube (VT) connected through a hose. This setup enhances the efficiency of interstitial fluid (ISF) extraction and facilitates rapid analysis. The VT, which applies negative pressure, allows the MAP to collect approximately 18 μL of ISF from the dermis of a live rabbit ear within 5 minutes. This method enables immediate and minimally invasive glucose measurement using personal healthcare meters. The VT-MAP system is user-friendly and can be integrated into a comprehensive and mobile ISF analysis system by preloading the hose with custom sensing papers for specific analytes. The device's high extraction rate and ease of use make it suitable for decentralized personal healthcare, particularly for monitoring glucose levels and other analytes in the skin. The study demonstrates the potential of this integrated system to revolutionize POCT applications, providing a reliable and accessible alternative to traditional blood sampling methods.The paper introduces an innovative strategy for point-of-care testing (POCT) using a 3D-printed hollow microneedle array patch (MAP) and a vacuum tube (VT) connected through a hose. This setup enhances the efficiency of interstitial fluid (ISF) extraction and facilitates rapid analysis. The VT, which applies negative pressure, allows the MAP to collect approximately 18 μL of ISF from the dermis of a live rabbit ear within 5 minutes. This method enables immediate and minimally invasive glucose measurement using personal healthcare meters. The VT-MAP system is user-friendly and can be integrated into a comprehensive and mobile ISF analysis system by preloading the hose with custom sensing papers for specific analytes. The device's high extraction rate and ease of use make it suitable for decentralized personal healthcare, particularly for monitoring glucose levels and other analytes in the skin. The study demonstrates the potential of this integrated system to revolutionize POCT applications, providing a reliable and accessible alternative to traditional blood sampling methods.