This study presents the enhanced functionalization of Mn₂O₃@SiO₂ core-shell nanostructures using hydrolysis of organosilane precursors. The synthesis involves dispersing Mn₂O₃ nanoparticles in a Tergitol/cyclohexane mixture and hydrolyzing TEOS or functionalized organosilanes to form silica shells. The method ensures a high density of functional groups on the nanostructures, which can be used for immobilizing biomolecules and drugs, as well as for trace element extraction. The nanostructures were characterized using TEM, IR, and zeta potential studies. The zeta potential results showed that the hydrolysis method produced more functional groups compared to post-grafting. Amino-functionalized nanostructures were used to immobilize glucose and L-methionine, with zeta potential studies confirming successful immobilization. The method is an improvement over traditional post-grafting or co-condensation techniques, as it allows for more functional groups on the nanostructures. The study also demonstrates the potential of these nanostructures for biomedical and catalytic applications. The results show that the hydrolysis method leads to higher surface charge density and more functional groups on the nanostructures compared to the post-grafting method. The study highlights the effectiveness of the hydrolysis method in functionalizing core-shell nanostructures for various applications.This study presents the enhanced functionalization of Mn₂O₃@SiO₂ core-shell nanostructures using hydrolysis of organosilane precursors. The synthesis involves dispersing Mn₂O₃ nanoparticles in a Tergitol/cyclohexane mixture and hydrolyzing TEOS or functionalized organosilanes to form silica shells. The method ensures a high density of functional groups on the nanostructures, which can be used for immobilizing biomolecules and drugs, as well as for trace element extraction. The nanostructures were characterized using TEM, IR, and zeta potential studies. The zeta potential results showed that the hydrolysis method produced more functional groups compared to post-grafting. Amino-functionalized nanostructures were used to immobilize glucose and L-methionine, with zeta potential studies confirming successful immobilization. The method is an improvement over traditional post-grafting or co-condensation techniques, as it allows for more functional groups on the nanostructures. The study also demonstrates the potential of these nanostructures for biomedical and catalytic applications. The results show that the hydrolysis method leads to higher surface charge density and more functional groups on the nanostructures compared to the post-grafting method. The study highlights the effectiveness of the hydrolysis method in functionalizing core-shell nanostructures for various applications.