The study demonstrates that a TMPRSS2-expressing VeroE6 cell line is highly susceptible to SARS-CoV-2 infection, making it useful for isolating and propagating the virus. The researchers found that SARS-CoV-2 infection is enhanced by TMPRSS2, similar to SARS-CoV and MERS-CoV. The VeroE6/TMPRSS2 cell line showed significantly higher viral RNA copies and infected cells compared to other cell lines. The findings suggest that TMPRSS2 may play a crucial role in SARS-CoV-2 entry and replication, highlighting its potential as a target for therapeutic agents. The VeroE6/TMPRSS2 cell line is now available from the Japanese Collection of Research Bioresources (JCRB) Cell Bank.The study demonstrates that a TMPRSS2-expressing VeroE6 cell line is highly susceptible to SARS-CoV-2 infection, making it useful for isolating and propagating the virus. The researchers found that SARS-CoV-2 infection is enhanced by TMPRSS2, similar to SARS-CoV and MERS-CoV. The VeroE6/TMPRSS2 cell line showed significantly higher viral RNA copies and infected cells compared to other cell lines. The findings suggest that TMPRSS2 may play a crucial role in SARS-CoV-2 entry and replication, highlighting its potential as a target for therapeutic agents. The VeroE6/TMPRSS2 cell line is now available from the Japanese Collection of Research Bioresources (JCRB) Cell Bank.