This study investigates the role of flavonoids in enhancing oxidative and drought stress tolerance in Arabidopsis thaliana. The researchers overexpressed the flavonoid-specific transcription factors MYB12 and PAP1, leading to increased flavonoid accumulation. Metabolome and transcriptome profiling, as well as stress experiments, were conducted to assess the effects of flavonoid overaccumulation. The results showed that flavonoid overexpression significantly increased the levels of flavonols and anthocyanins, which exhibited strong radical scavenging activity in vitro. In vivo, these flavonoids mitigated the accumulation of reactive oxygen species (ROS) under oxidative and drought stress conditions. Additionally, the flavonoid-deficient mutant lines did not show enhanced stress tolerance, confirming the importance of flavonoids in stress response. The study also found that flavonoid accumulation prevented water loss in whole plants and detached leaves, suggesting a role in maintaining water homeostasis. These findings highlight the potential of flavonoids as antioxidants and stress protectants in crops, offering insights into enhancing crop resilience to environmental stresses.This study investigates the role of flavonoids in enhancing oxidative and drought stress tolerance in Arabidopsis thaliana. The researchers overexpressed the flavonoid-specific transcription factors MYB12 and PAP1, leading to increased flavonoid accumulation. Metabolome and transcriptome profiling, as well as stress experiments, were conducted to assess the effects of flavonoid overaccumulation. The results showed that flavonoid overexpression significantly increased the levels of flavonols and anthocyanins, which exhibited strong radical scavenging activity in vitro. In vivo, these flavonoids mitigated the accumulation of reactive oxygen species (ROS) under oxidative and drought stress conditions. Additionally, the flavonoid-deficient mutant lines did not show enhanced stress tolerance, confirming the importance of flavonoids in stress response. The study also found that flavonoid accumulation prevented water loss in whole plants and detached leaves, suggesting a role in maintaining water homeostasis. These findings highlight the potential of flavonoids as antioxidants and stress protectants in crops, offering insights into enhancing crop resilience to environmental stresses.