The study investigates the coupling of a single molecule to a gold nanoparticle (GNP) acting as a nano-antenna, using scanning probe technology to position the GNP with nanometer accuracy. The results show a significant enhancement of fluorescence intensity by more than 20 times and a 20-fold shortening of the excited state lifetime. The enhancements are attributed to the excitation enhancement, modification of spontaneous emission, and quenching, with the plasmon resonance of the GNP playing a crucial role in modifying the molecular emission. The findings are supported by three-dimensional calculations, which reveal the contributions of various factors to the observed effects. The study also demonstrates the potential of this approach for applications such as detecting weakly fluorescing systems.The study investigates the coupling of a single molecule to a gold nanoparticle (GNP) acting as a nano-antenna, using scanning probe technology to position the GNP with nanometer accuracy. The results show a significant enhancement of fluorescence intensity by more than 20 times and a 20-fold shortening of the excited state lifetime. The enhancements are attributed to the excitation enhancement, modification of spontaneous emission, and quenching, with the plasmon resonance of the GNP playing a crucial role in modifying the molecular emission. The findings are supported by three-dimensional calculations, which reveal the contributions of various factors to the observed effects. The study also demonstrates the potential of this approach for applications such as detecting weakly fluorescing systems.