CRISPR/Cas gene editing technology has been increasingly used to enhance specialized metabolites in medicinal plants. This review summarizes the latest advancements in the application of CRISPR/Cas gene editing for the purpose of augmenting specific metabolites in medicinal plants. The review discusses the mechanisms of CRISPR/Cas gene editing, including the use of single-guide RNA (sgRNA) and the CRISPR/Cas9 system. It also highlights the current status of plant metabolic engineering in the CRISPR era, focusing on the enhancement of specialized metabolites in crops such as rice, tomato, and wheat. The review also discusses the application of CRISPR/Cas gene editing in medicinal plants to increase the production of specialty metabolites, including the use of CRISPR/Cas9 for targeted mutagenesis and knockout of genes involved in the biosynthesis of specialized metabolites. The review also discusses the potential of CRISPR/Cas gene editing in the development of medicinal plants with enhanced therapeutic properties. The review concludes that CRISPR/Cas gene editing has the potential to significantly enhance the production of specialized metabolites in medicinal plants, leading to improved therapeutic applications and commercial value.CRISPR/Cas gene editing technology has been increasingly used to enhance specialized metabolites in medicinal plants. This review summarizes the latest advancements in the application of CRISPR/Cas gene editing for the purpose of augmenting specific metabolites in medicinal plants. The review discusses the mechanisms of CRISPR/Cas gene editing, including the use of single-guide RNA (sgRNA) and the CRISPR/Cas9 system. It also highlights the current status of plant metabolic engineering in the CRISPR era, focusing on the enhancement of specialized metabolites in crops such as rice, tomato, and wheat. The review also discusses the application of CRISPR/Cas gene editing in medicinal plants to increase the production of specialty metabolites, including the use of CRISPR/Cas9 for targeted mutagenesis and knockout of genes involved in the biosynthesis of specialized metabolites. The review also discusses the potential of CRISPR/Cas gene editing in the development of medicinal plants with enhanced therapeutic properties. The review concludes that CRISPR/Cas gene editing has the potential to significantly enhance the production of specialized metabolites in medicinal plants, leading to improved therapeutic applications and commercial value.