This review discusses the potential of enzymatic biofuel cells (EBFCs) as a power source for self-sustained smart textiles. EBFCs, which convert chemical energy from physiological glucose or lactate into electricity, offer several advantages over traditional lithium-ion batteries, including continuous power generation, biocompatibility, simple configuration, and biodegradability. The review highlights the recent progress in EBFCs, focusing on their application in fibers, yarns, and textiles. It also addresses the challenges and perspectives for further development, emphasizing the need for interdisciplinary efforts to integrate EBFCs into smart textile systems. The review concludes by discussing the performance limitations of current EBFCs and the potential for combining EBFCs with other energy-harvesting devices to enhance power output and sustainability.This review discusses the potential of enzymatic biofuel cells (EBFCs) as a power source for self-sustained smart textiles. EBFCs, which convert chemical energy from physiological glucose or lactate into electricity, offer several advantages over traditional lithium-ion batteries, including continuous power generation, biocompatibility, simple configuration, and biodegradability. The review highlights the recent progress in EBFCs, focusing on their application in fibers, yarns, and textiles. It also addresses the challenges and perspectives for further development, emphasizing the need for interdisciplinary efforts to integrate EBFCs into smart textile systems. The review concludes by discussing the performance limitations of current EBFCs and the potential for combining EBFCs with other energy-harvesting devices to enhance power output and sustainability.