The article reviews the enzymatic degradation of polylactic acid (PLA), a biodegradable polyester produced from renewable resources. PLA's environmental benefits and wide range of applications have led to its increasing use, but its insufficient degradability under natural conditions highlights the need for improved biodegradation techniques. The review focuses on various enzymes that can degrade PLA, including proteases, lipases, cutinases, and esterases, which are secreted by different microorganisms. It details the characteristics of these enzymes, recent advances, and methods for evaluating their activity and enantiopurity. While complete degradation of solid PLA particles remains challenging, future research and improvements in enzyme properties may lead to advanced procedures for PLA degradation and upcycling, aligning with circular economy principles. The article also discusses the impact of polymer properties, reaction conditions, and microbial degradation mechanisms on PLA degradation rates, emphasizing the potential for enhancing enzymatic degradation through protein engineering and machine learning techniques.The article reviews the enzymatic degradation of polylactic acid (PLA), a biodegradable polyester produced from renewable resources. PLA's environmental benefits and wide range of applications have led to its increasing use, but its insufficient degradability under natural conditions highlights the need for improved biodegradation techniques. The review focuses on various enzymes that can degrade PLA, including proteases, lipases, cutinases, and esterases, which are secreted by different microorganisms. It details the characteristics of these enzymes, recent advances, and methods for evaluating their activity and enantiopurity. While complete degradation of solid PLA particles remains challenging, future research and improvements in enzyme properties may lead to advanced procedures for PLA degradation and upcycling, aligning with circular economy principles. The article also discusses the impact of polymer properties, reaction conditions, and microbial degradation mechanisms on PLA degradation rates, emphasizing the potential for enhancing enzymatic degradation through protein engineering and machine learning techniques.