The study identifies a novel cardiogenic precursor, marked by the transcription factor Wt1, located within the epicardium. During normal heart development, a subset of these Wt1-expressing cells differentiate into fully functional cardiomyocytes. These Wt1+ proepicardial cells arise from progenitors that express Nkx2-5 and Isl1, suggesting a shared developmental origin with multipotent Nkx2-5+/Isl1+ progenitors. The findings identify Wt1+ epicardial cells as previously unrecognized cardiomyocyte progenitors, providing a foundation for future efforts to harness their cardiogenic potential for cardiac regeneration and repair. The study also demonstrates that Wt1+ cells in the epicardium differentiate into cardiomyocytes, and that this process is temporally and spatially regulated. Additionally, the study shows that Wt1+ cells in the proepicardium are derived from Nkx2-5-expressing precursors, suggesting a sequential or transient co-expression of these transcription factors.The study identifies a novel cardiogenic precursor, marked by the transcription factor Wt1, located within the epicardium. During normal heart development, a subset of these Wt1-expressing cells differentiate into fully functional cardiomyocytes. These Wt1+ proepicardial cells arise from progenitors that express Nkx2-5 and Isl1, suggesting a shared developmental origin with multipotent Nkx2-5+/Isl1+ progenitors. The findings identify Wt1+ epicardial cells as previously unrecognized cardiomyocyte progenitors, providing a foundation for future efforts to harness their cardiogenic potential for cardiac regeneration and repair. The study also demonstrates that Wt1+ cells in the epicardium differentiate into cardiomyocytes, and that this process is temporally and spatially regulated. Additionally, the study shows that Wt1+ cells in the proepicardium are derived from Nkx2-5-expressing precursors, suggesting a sequential or transient co-expression of these transcription factors.