This review summarizes the physiological responses of plants to salinity stress, focusing on how to quantify and analyze the effects of salinity on various traits such as growth, water relations, ion homeostasis, photosynthesis, yield components, and senescence. The authors emphasize the importance of selecting appropriate experimental systems, applying salinity stress, and using suitable indices to assess physiological data. They suggest using graphs to correlate traits like leaf Na content with salinity tolerance indices to identify the most important contributors to salinity tolerance. The review also discusses the complexity of salinity tolerance, involving multiple genes and mechanisms, and highlights the need for improved experimental design in previous studies. The authors provide guidelines for designing experiments and analyzing data related to salinity tolerance, including the use of destructive and non-destructive methods, such as image analysis and dose-response curves. They also discuss the importance of measuring ion relations, photosynthesis, and senescence in assessing salinity tolerance. The review concludes that understanding the physiological responses of plants to salinity is crucial for developing salt-tolerant crops and improving agricultural productivity under saline conditions.This review summarizes the physiological responses of plants to salinity stress, focusing on how to quantify and analyze the effects of salinity on various traits such as growth, water relations, ion homeostasis, photosynthesis, yield components, and senescence. The authors emphasize the importance of selecting appropriate experimental systems, applying salinity stress, and using suitable indices to assess physiological data. They suggest using graphs to correlate traits like leaf Na content with salinity tolerance indices to identify the most important contributors to salinity tolerance. The review also discusses the complexity of salinity tolerance, involving multiple genes and mechanisms, and highlights the need for improved experimental design in previous studies. The authors provide guidelines for designing experiments and analyzing data related to salinity tolerance, including the use of destructive and non-destructive methods, such as image analysis and dose-response curves. They also discuss the importance of measuring ion relations, photosynthesis, and senescence in assessing salinity tolerance. The review concludes that understanding the physiological responses of plants to salinity is crucial for developing salt-tolerant crops and improving agricultural productivity under saline conditions.