This review by Negrão, Schmöckel, and Tester discusses the evaluation of plant physiological responses to salinity stress, a major abiotic constraint affecting crop yield. The authors highlight the complexity of salinity stress, which involves multiple physiological responses, and provide guidelines for quantifying these responses in different traits such as growth, water relations, ion homeostasis, photosynthesis, yield components, and senescence. They emphasize the importance of using appropriate experimental systems, imposing salinity stress effectively, and analyzing physiological data using relevant indices. The review also addresses the challenges in interpreting data and suggests methods for data presentation and analysis, including correlation plots and principal component analysis (PCA). The authors aim to assist researchers in conducting proficient assays and interpreting results from salinity tolerance experiments, emphasizing the need for rigorous experimental methodologies.This review by Negrão, Schmöckel, and Tester discusses the evaluation of plant physiological responses to salinity stress, a major abiotic constraint affecting crop yield. The authors highlight the complexity of salinity stress, which involves multiple physiological responses, and provide guidelines for quantifying these responses in different traits such as growth, water relations, ion homeostasis, photosynthesis, yield components, and senescence. They emphasize the importance of using appropriate experimental systems, imposing salinity stress effectively, and analyzing physiological data using relevant indices. The review also addresses the challenges in interpreting data and suggests methods for data presentation and analysis, including correlation plots and principal component analysis (PCA). The authors aim to assist researchers in conducting proficient assays and interpreting results from salinity tolerance experiments, emphasizing the need for rigorous experimental methodologies.