Empagliflozin (EMP) was evaluated for its potential as an anti-aging treatment in mice. The study involved four groups of mice: a negative control group receiving normal saline, and three treatment groups receiving D-galactose (DGA) for eight weeks, followed by either vitamin C or EMP. The results showed that EMP significantly reduced levels of TNF-α, IL-1β, and MDA compared to the DGA group, while increasing GSH-Px levels. Additionally, EMP increased the levels of COL-1 and COL-3 compared to the DGA group. These findings suggest that EMP positively affects several aging parameters in mice, including reducing inflammation, oxidative stress, and collagen degradation. The study also found that EMP reduced heart weight and improved heart function, indicating its potential to mitigate age-related cardiac changes. EMP's anti-inflammatory and antioxidant properties were attributed to its ability to inhibit COX-2, iNOS, and other inflammatory pathways. The study concluded that EMP may have a beneficial effect on aging by reducing oxidative stress and inflammation, and improving collagen levels in the skin. However, the study had limitations, including the focus on only two organs and the inability to assess genetic and molecular pathways. Overall, the study supports the potential of EMP as a promising anti-aging treatment in mice.Empagliflozin (EMP) was evaluated for its potential as an anti-aging treatment in mice. The study involved four groups of mice: a negative control group receiving normal saline, and three treatment groups receiving D-galactose (DGA) for eight weeks, followed by either vitamin C or EMP. The results showed that EMP significantly reduced levels of TNF-α, IL-1β, and MDA compared to the DGA group, while increasing GSH-Px levels. Additionally, EMP increased the levels of COL-1 and COL-3 compared to the DGA group. These findings suggest that EMP positively affects several aging parameters in mice, including reducing inflammation, oxidative stress, and collagen degradation. The study also found that EMP reduced heart weight and improved heart function, indicating its potential to mitigate age-related cardiac changes. EMP's anti-inflammatory and antioxidant properties were attributed to its ability to inhibit COX-2, iNOS, and other inflammatory pathways. The study concluded that EMP may have a beneficial effect on aging by reducing oxidative stress and inflammation, and improving collagen levels in the skin. However, the study had limitations, including the focus on only two organs and the inability to assess genetic and molecular pathways. Overall, the study supports the potential of EMP as a promising anti-aging treatment in mice.