Metformin, a widely used drug for type 2 diabetes, has been investigated for its potential antioxidant properties. This review explores the clinical relevance of metformin as an antioxidant intervention, highlighting its mechanisms of action, including modulation of oxidative stress, activation of AMPK and SIRT3 pathways, and inhibition of NADPH oxidase. Metformin has been shown to reduce oxidative stress markers such as malondialdehyde and advanced oxidation protein products, and to enhance antioxidant enzyme activities. In vitro and in vivo studies suggest that metformin can reduce reactive oxygen species (ROS) production and improve cellular antioxidant defenses. However, the results of clinical trials are inconsistent, with some studies showing that metformin reduces oxidative stress while others indicate no significant effect. The effectiveness of metformin as an antioxidant may depend on factors such as dosage, treatment duration, and patient characteristics. Despite these variations, metformin remains a well-tolerated and effective drug for managing type 2 diabetes, with potential benefits extending beyond glycemic control. Further research is needed to fully understand the antioxidant properties of metformin and to determine its clinical utility in various disease states.Metformin, a widely used drug for type 2 diabetes, has been investigated for its potential antioxidant properties. This review explores the clinical relevance of metformin as an antioxidant intervention, highlighting its mechanisms of action, including modulation of oxidative stress, activation of AMPK and SIRT3 pathways, and inhibition of NADPH oxidase. Metformin has been shown to reduce oxidative stress markers such as malondialdehyde and advanced oxidation protein products, and to enhance antioxidant enzyme activities. In vitro and in vivo studies suggest that metformin can reduce reactive oxygen species (ROS) production and improve cellular antioxidant defenses. However, the results of clinical trials are inconsistent, with some studies showing that metformin reduces oxidative stress while others indicate no significant effect. The effectiveness of metformin as an antioxidant may depend on factors such as dosage, treatment duration, and patient characteristics. Despite these variations, metformin remains a well-tolerated and effective drug for managing type 2 diabetes, with potential benefits extending beyond glycemic control. Further research is needed to fully understand the antioxidant properties of metformin and to determine its clinical utility in various disease states.