Exosomes, naturally occurring nanovesicles, have gained attention as potential drug delivery vehicles. This study explores the use of exosomes secreted by monocytes and macrophages to enhance the delivery of catalase, a potent antioxidant, to treat Parkinson's disease (PD). Catalase was loaded into exosomes using various methods, including incubation at room temperature, freeze-thaw cycles, sonication, and extrusion. The resulting exoCAT formulations showed high loading efficiency, sustained release, and preservation of catalase activity. In vitro and in vivo models of PD demonstrated that exoCAT significantly reduced oxidative stress and increased neuronal survival. The study highlights the potential of exosome-based catalase formulations as a versatile strategy for treating inflammatory and neurodegenerative disorders, particularly PD.Exosomes, naturally occurring nanovesicles, have gained attention as potential drug delivery vehicles. This study explores the use of exosomes secreted by monocytes and macrophages to enhance the delivery of catalase, a potent antioxidant, to treat Parkinson's disease (PD). Catalase was loaded into exosomes using various methods, including incubation at room temperature, freeze-thaw cycles, sonication, and extrusion. The resulting exoCAT formulations showed high loading efficiency, sustained release, and preservation of catalase activity. In vitro and in vivo models of PD demonstrated that exoCAT significantly reduced oxidative stress and increased neuronal survival. The study highlights the potential of exosome-based catalase formulations as a versatile strategy for treating inflammatory and neurodegenerative disorders, particularly PD.