A cross-sectional study evaluated the pituitary function in patients previously infected with SARS-CoV-2, comparing those who had fully recovered (G1) with those experiencing long COVID (G2). The study aimed to identify potential anterior pituitary deficiencies that may contribute to long COVID symptoms. A total of 64 patients were assessed, with 32 in each group. G2 showed significantly higher rates of corticotroph and somatotroph deficiencies compared to G1. Corticotroph deficiencies were observed in 28.1% of G2 versus 6.3% of G1, while somatotroph deficiencies were 59.4% in G2 versus 31.3% in G1. Baseline and peak cortisol levels were lower in G2, and peak growth hormone levels were also lower in G2. These findings support the endocrine hypothesis, suggesting that anterior pituitary insufficiency may play a role in long COVID. The study highlights the importance of evaluating pituitary function in long COVID patients, as deficiencies in corticotroph and somatotroph axes may contribute to persistent symptoms. The results indicate that long COVID patients are more likely to have anterior pituitary hormone deficiencies, which could explain some of the symptoms associated with the condition. The study is the first to use an insulin tolerance test (ITT) to assess the corticotroph and somatotroph axes in a large cohort of long COVID patients. The findings suggest that further research is needed to establish the link between long COVID and anterior pituitary deficiencies, which could lead to improved management and diagnostic guidelines for the condition.A cross-sectional study evaluated the pituitary function in patients previously infected with SARS-CoV-2, comparing those who had fully recovered (G1) with those experiencing long COVID (G2). The study aimed to identify potential anterior pituitary deficiencies that may contribute to long COVID symptoms. A total of 64 patients were assessed, with 32 in each group. G2 showed significantly higher rates of corticotroph and somatotroph deficiencies compared to G1. Corticotroph deficiencies were observed in 28.1% of G2 versus 6.3% of G1, while somatotroph deficiencies were 59.4% in G2 versus 31.3% in G1. Baseline and peak cortisol levels were lower in G2, and peak growth hormone levels were also lower in G2. These findings support the endocrine hypothesis, suggesting that anterior pituitary insufficiency may play a role in long COVID. The study highlights the importance of evaluating pituitary function in long COVID patients, as deficiencies in corticotroph and somatotroph axes may contribute to persistent symptoms. The results indicate that long COVID patients are more likely to have anterior pituitary hormone deficiencies, which could explain some of the symptoms associated with the condition. The study is the first to use an insulin tolerance test (ITT) to assess the corticotroph and somatotroph axes in a large cohort of long COVID patients. The findings suggest that further research is needed to establish the link between long COVID and anterior pituitary deficiencies, which could lead to improved management and diagnostic guidelines for the condition.