This review explores the synthesis, characteristics, and wide-ranging applications of hyaluronic acid (HA) hydrogels. HA hydrogels are biocompatible, biodegradable, and capable of mimicking the extracellular matrix (ECM), making them ideal for biomedical applications such as tissue engineering, drug delivery, wound healing, and regenerative medicine. The review discusses various cross-linking methods, including chemical, physical, enzymatic, and hybrid techniques, which influence the mechanical strength, swelling capacity, and degradation rate of HA hydrogels. Assessment tools like rheological analysis, electron microscopy, spectroscopy, swelling tests, and degradation studies are used to evaluate their properties. HA-based hydrogels are widely used in tissue engineering, drug distribution, wound recovery, ophthalmology, and cartilage repair. The synthesis method affects the hydrogel's mechanical properties, swelling behavior, and degradation rate. The review highlights the importance of HA hydrogels in biomedical applications and provides a comprehensive overview of their synthesis, characterization, and potential uses.This review explores the synthesis, characteristics, and wide-ranging applications of hyaluronic acid (HA) hydrogels. HA hydrogels are biocompatible, biodegradable, and capable of mimicking the extracellular matrix (ECM), making them ideal for biomedical applications such as tissue engineering, drug delivery, wound healing, and regenerative medicine. The review discusses various cross-linking methods, including chemical, physical, enzymatic, and hybrid techniques, which influence the mechanical strength, swelling capacity, and degradation rate of HA hydrogels. Assessment tools like rheological analysis, electron microscopy, spectroscopy, swelling tests, and degradation studies are used to evaluate their properties. HA-based hydrogels are widely used in tissue engineering, drug distribution, wound recovery, ophthalmology, and cartilage repair. The synthesis method affects the hydrogel's mechanical properties, swelling behavior, and degradation rate. The review highlights the importance of HA hydrogels in biomedical applications and provides a comprehensive overview of their synthesis, characterization, and potential uses.