This study presents a green, extracellular, and sustainable method for synthesizing silver nanoparticles (AgNPs) using cell-free yeast extract. The yeast extract serves as a renewable and biogenic source for reducing silver precursors and stabilizing AgNPs. The method is cost-effective, environmentally friendly, and scalable, offering advantages over traditional intracellular methods. HR-TEM imaging revealed isotropic growth of AgNPs with an average size of about 18 nm, ranging from spherical to oval. FTIR and XPS analysis identified functional groups such as carboxyl, hydroxyl, and amide, contributing to colloidal stability. AgNPs exhibited potent antibacterial activity against multidrug-resistant (MDR) bacteria, particularly Gram-negative strains. Seed priming experiments showed that AgNPs improved germination rates and survival of Sorghum jowar and Zea mays seedlings. The study highlights the potential of AgNPs as alternative antimicrobial agents and seed priming agents. The biosynthesis process was optimized using parameters such as pH, yeast extract concentration, and AgNO3 concentration. The results demonstrate the effectiveness of AgNPs in combating MDR bacteria and enhancing seed germination. The method aligns with green chemistry principles, offering a sustainable and efficient approach for AgNP synthesis. The findings suggest that AgNPs can be used in various applications, including antibacterial treatments and agricultural seed priming. The study underscores the importance of optimizing reaction parameters to achieve high-quality, uniform AgNPs with desired physicochemical properties. The results indicate that AgNPs can serve as a safe and effective alternative to conventional antibiotics and chemical treatments in agriculture. The study provides valuable insights into the potential of AgNPs in addressing antimicrobial resistance and improving agricultural practices.This study presents a green, extracellular, and sustainable method for synthesizing silver nanoparticles (AgNPs) using cell-free yeast extract. The yeast extract serves as a renewable and biogenic source for reducing silver precursors and stabilizing AgNPs. The method is cost-effective, environmentally friendly, and scalable, offering advantages over traditional intracellular methods. HR-TEM imaging revealed isotropic growth of AgNPs with an average size of about 18 nm, ranging from spherical to oval. FTIR and XPS analysis identified functional groups such as carboxyl, hydroxyl, and amide, contributing to colloidal stability. AgNPs exhibited potent antibacterial activity against multidrug-resistant (MDR) bacteria, particularly Gram-negative strains. Seed priming experiments showed that AgNPs improved germination rates and survival of Sorghum jowar and Zea mays seedlings. The study highlights the potential of AgNPs as alternative antimicrobial agents and seed priming agents. The biosynthesis process was optimized using parameters such as pH, yeast extract concentration, and AgNO3 concentration. The results demonstrate the effectiveness of AgNPs in combating MDR bacteria and enhancing seed germination. The method aligns with green chemistry principles, offering a sustainable and efficient approach for AgNP synthesis. The findings suggest that AgNPs can be used in various applications, including antibacterial treatments and agricultural seed priming. The study underscores the importance of optimizing reaction parameters to achieve high-quality, uniform AgNPs with desired physicochemical properties. The results indicate that AgNPs can serve as a safe and effective alternative to conventional antibiotics and chemical treatments in agriculture. The study provides valuable insights into the potential of AgNPs in addressing antimicrobial resistance and improving agricultural practices.