This study introduces a green, extracellular, and sustainable methodology for the bio-materialization of silver nanoparticles (AgNPs) using renewable cell-free yeast extracts. The method offers several advantages, including cost-effectiveness, environmental friendliness, ease of synthesis, and scalability. HR-TEM imaging revealed that the biosynthesized AgNPs had an average size of about 18 nm and varied in shape from spherical to oval. FTIR and XPS results showed the presence of functional groups such as carboxyl, hydroxyl, and amide, which contribute to enhanced colloidal stability. AgNPs exhibited potent antibacterial activity against multidrug-resistant (MDR) strains, particularly against Gram-negative bacteria. Seed priming experiments demonstrated that pre-sowing treatment with AgNPs improved both the germination rate and survival of Sorghum jowar and Zea mays seedlings. The study highlights the potential of AgNPs as a promising alternative to traditional antibiotics in addressing the growing threat of antimicrobial resistance and as a sustainable seed priming agent in agriculture.This study introduces a green, extracellular, and sustainable methodology for the bio-materialization of silver nanoparticles (AgNPs) using renewable cell-free yeast extracts. The method offers several advantages, including cost-effectiveness, environmental friendliness, ease of synthesis, and scalability. HR-TEM imaging revealed that the biosynthesized AgNPs had an average size of about 18 nm and varied in shape from spherical to oval. FTIR and XPS results showed the presence of functional groups such as carboxyl, hydroxyl, and amide, which contribute to enhanced colloidal stability. AgNPs exhibited potent antibacterial activity against multidrug-resistant (MDR) strains, particularly against Gram-negative bacteria. Seed priming experiments demonstrated that pre-sowing treatment with AgNPs improved both the germination rate and survival of Sorghum jowar and Zea mays seedlings. The study highlights the potential of AgNPs as a promising alternative to traditional antibiotics in addressing the growing threat of antimicrobial resistance and as a sustainable seed priming agent in agriculture.