A study was conducted to extract, characterize, and evaluate the properties of pineapple leaf fibers (PALF) from Azores pineapples. The research aimed to address the lack of technology for PALF extraction and treatment in Europe. Long fibers were extracted using hand scraping and biological retting at 25°C for different periods. The properties of PALF from plants of different ages (11- and 18-months) and different leaf positions (beginning, middle, and tip) were evaluated. Physical-mechanical properties, including diameter, linear density, strength, Young's modulus, and elongation at break, were determined. Chemical and morphological characteristics were analyzed using ATR-FTIR, XRD, TGA/DTG, and FESEM. Biological retting for 14 days effectively removed non-fibrous matter, producing the thinnest and strongest fibers. These fibers ranged from 34.9 to 168.3 µm in diameter, 1.39 to 7.07 tex in linear density, 37–993 MPa in tensile strength, 1.0–3.9% in elongation at break, and 2.4–21.8 GPa in Young's modulus. The study also explored the effects of extraction methods, plant age, and leaf position on fiber properties. The results showed that biological retting at 25°C for 14 days yielded the best mechanical properties. The crystallinity index of cellulose was similar across different extraction methods, indicating preservation of hemicellulose and lignin. The findings suggest that Azores pineapple waste is a valuable resource for the textile industry, contributing to a circular economy and providing high-quality fibers for various applications. The study highlights the potential of PALF in textiles, composites, and other materials, emphasizing the importance of optimizing extraction methods to enhance fiber properties.A study was conducted to extract, characterize, and evaluate the properties of pineapple leaf fibers (PALF) from Azores pineapples. The research aimed to address the lack of technology for PALF extraction and treatment in Europe. Long fibers were extracted using hand scraping and biological retting at 25°C for different periods. The properties of PALF from plants of different ages (11- and 18-months) and different leaf positions (beginning, middle, and tip) were evaluated. Physical-mechanical properties, including diameter, linear density, strength, Young's modulus, and elongation at break, were determined. Chemical and morphological characteristics were analyzed using ATR-FTIR, XRD, TGA/DTG, and FESEM. Biological retting for 14 days effectively removed non-fibrous matter, producing the thinnest and strongest fibers. These fibers ranged from 34.9 to 168.3 µm in diameter, 1.39 to 7.07 tex in linear density, 37–993 MPa in tensile strength, 1.0–3.9% in elongation at break, and 2.4–21.8 GPa in Young's modulus. The study also explored the effects of extraction methods, plant age, and leaf position on fiber properties. The results showed that biological retting at 25°C for 14 days yielded the best mechanical properties. The crystallinity index of cellulose was similar across different extraction methods, indicating preservation of hemicellulose and lignin. The findings suggest that Azores pineapple waste is a valuable resource for the textile industry, contributing to a circular economy and providing high-quality fibers for various applications. The study highlights the potential of PALF in textiles, composites, and other materials, emphasizing the importance of optimizing extraction methods to enhance fiber properties.