The authors have developed a DNA extraction method for milligram amounts of plant tissue, yielding 0.3–200 nanograms of DNA per milligram of tissue. The method, based on cetyltrimethylammonium bromide (CTAB) nucleic acid extraction, is efficient and does not require expensive equipment or time-consuming procedures. The technique was successfully applied to various fresh, herbarium, and mummified plant tissues, including seeds and embryos, with no observed inhibition for restriction enzymes BamHI or EcoRI. The study involved 57 types from 29 species, demonstrating the versatility and effectiveness of the method. The detailed protocol includes tissue grinding, buffer addition, chloroform extraction, and final precipitation steps, ensuring high yields of pure, high molecular weight DNA.The authors have developed a DNA extraction method for milligram amounts of plant tissue, yielding 0.3–200 nanograms of DNA per milligram of tissue. The method, based on cetyltrimethylammonium bromide (CTAB) nucleic acid extraction, is efficient and does not require expensive equipment or time-consuming procedures. The technique was successfully applied to various fresh, herbarium, and mummified plant tissues, including seeds and embryos, with no observed inhibition for restriction enzymes BamHI or EcoRI. The study involved 57 types from 29 species, demonstrating the versatility and effectiveness of the method. The detailed protocol includes tissue grinding, buffer addition, chloroform extraction, and final precipitation steps, ensuring high yields of pure, high molecular weight DNA.