The article discusses the use of freeze-etching, a specialized freeze-drying technique, to study the fine structure of yeast cells (Saccharomyces cerevisiae) under electron microscopy. This method allows for the investigation of cells in a frozen state without chemical fixation, preserving their natural structure. The technique involves freezing the cells, cutting them, and freeze-drying the cut surface to reveal detailed structural features. The resulting electron micrographs provide high-fidelity images of cellular components, including the cytoplasmic membrane, which contains hexagonal arrangements of particles possibly involved in glucan fibril production. The nuclear pores vary in distribution depending on the cell's age, and the endoplasmic reticulum and vacuoles can be clearly distinguished. The mitochondrial envelope has small perforations that may facilitate macromolecule exchange, while storage granules consist of concentric lipid layers. The Golgi apparatus is detected and may be involved in lipid storage. The unit membrane structure and other organelle membranes are confirmed. Freeze-etching also reveals glycogen agglomerations in older cells and provides insights into artifacts caused by chemical fixation. The method is compared to traditional chemical fixation, which often distorts structures due to shrinkage and washing out. The study highlights the advantages of freeze-etching in preserving cellular structures and provides detailed information on various organelles and their structures in yeast cells. The findings suggest that freeze-etching is a valuable technique for studying yeast cell structure without the distortions caused by chemical fixation.The article discusses the use of freeze-etching, a specialized freeze-drying technique, to study the fine structure of yeast cells (Saccharomyces cerevisiae) under electron microscopy. This method allows for the investigation of cells in a frozen state without chemical fixation, preserving their natural structure. The technique involves freezing the cells, cutting them, and freeze-drying the cut surface to reveal detailed structural features. The resulting electron micrographs provide high-fidelity images of cellular components, including the cytoplasmic membrane, which contains hexagonal arrangements of particles possibly involved in glucan fibril production. The nuclear pores vary in distribution depending on the cell's age, and the endoplasmic reticulum and vacuoles can be clearly distinguished. The mitochondrial envelope has small perforations that may facilitate macromolecule exchange, while storage granules consist of concentric lipid layers. The Golgi apparatus is detected and may be involved in lipid storage. The unit membrane structure and other organelle membranes are confirmed. Freeze-etching also reveals glycogen agglomerations in older cells and provides insights into artifacts caused by chemical fixation. The method is compared to traditional chemical fixation, which often distorts structures due to shrinkage and washing out. The study highlights the advantages of freeze-etching in preserving cellular structures and provides detailed information on various organelles and their structures in yeast cells. The findings suggest that freeze-etching is a valuable technique for studying yeast cell structure without the distortions caused by chemical fixation.