The freeze-etching technique, a form of freeze-drying, allows electron microscopic examination of cells and tissues in the frozen state. In yeast cells (Saccharomyces cerevisiae), a freeze-fixation technique was developed that does not kill the object, resulting in high-fidelity images. This method enables cross-sectional and surface views of membranes and organelles, revealing structures not visible with conventional sectioning. The cytoplasmic membrane contains hexagonal arrangements of particles involved in glucan fibril production. Nuclear pore distribution varies with cell age, and freeze-etching clearly distinguishes endoplasmic reticulum and vacuoles. Mitochondrial membranes have small perforations, and storage granules consist of lipid layers. A Golgi apparatus is detected, possibly involved in lipid storage. Freeze-etching confirms the structure of unit membranes and organelles, revealing glycogen agglomerations in older cells. Artifacts from chemical fixation are discussed. Freeze-etching provides detailed views of yeast cell structures, including the cell wall, cytoplasmic membrane, and nuclear envelope. The cell wall has a thickness of 700 Å, with invaginations from the cytoplasmic membrane. The cytoplasmic membrane has hexagonal particle arrangements and glucan fibrils. The nucleus is surrounded by two membranes with perforations. The perinuclear space is filled with water. Vacuoles and endoplasmic reticulum are distinct organelles. Mitochondria have a structure similar to those in higher plants, with two membranes and cristae. Lipid granules have a multilayered structure, possibly involved in lipid storage. The Golgi apparatus is present, possibly involved in lipid storage. Freeze-etching reveals the fine structure of yeast cells, providing insights into their morphology and function.The freeze-etching technique, a form of freeze-drying, allows electron microscopic examination of cells and tissues in the frozen state. In yeast cells (Saccharomyces cerevisiae), a freeze-fixation technique was developed that does not kill the object, resulting in high-fidelity images. This method enables cross-sectional and surface views of membranes and organelles, revealing structures not visible with conventional sectioning. The cytoplasmic membrane contains hexagonal arrangements of particles involved in glucan fibril production. Nuclear pore distribution varies with cell age, and freeze-etching clearly distinguishes endoplasmic reticulum and vacuoles. Mitochondrial membranes have small perforations, and storage granules consist of lipid layers. A Golgi apparatus is detected, possibly involved in lipid storage. Freeze-etching confirms the structure of unit membranes and organelles, revealing glycogen agglomerations in older cells. Artifacts from chemical fixation are discussed. Freeze-etching provides detailed views of yeast cell structures, including the cell wall, cytoplasmic membrane, and nuclear envelope. The cell wall has a thickness of 700 Å, with invaginations from the cytoplasmic membrane. The cytoplasmic membrane has hexagonal particle arrangements and glucan fibrils. The nucleus is surrounded by two membranes with perforations. The perinuclear space is filled with water. Vacuoles and endoplasmic reticulum are distinct organelles. Mitochondria have a structure similar to those in higher plants, with two membranes and cristae. Lipid granules have a multilayered structure, possibly involved in lipid storage. The Golgi apparatus is present, possibly involved in lipid storage. Freeze-etching reveals the fine structure of yeast cells, providing insights into their morphology and function.