19 February 2024 | Ricardo A. González-Hernández, Norma A. Valdez-Cruz, Mauricio A. Trujillo-Roldán
This review discusses the factors influencing the extraction methods of terpenes and terpenoids from natural sources. Terpenes and terpenoids, secondary metabolites found in various organisms, are of industrial interest due to their diverse applications. The extraction process is influenced by several factors, including drying methods, which can affect the recovery and quality of terpenes. Soxhlet extraction is commonly used due to its high efficiency, but unconventional methods like microwave and high-pressure extraction, as well as unconventional solvents, have shown promising results. The review also covers the preparation of biological material, including the selection of appropriate tissue and the importance of fragmentation methods. Dry and wet fragmentation methods are discussed, with dry methods being more cost-effective but requiring careful moisture control. Wet methods preserve the quality of the final product and are useful for recovering high-polarity molecules. The choice of extraction methods, such as steam distillation, adsorption-based techniques, and organic solvents, is crucial for recovering volatile and non-volatile terpenoids. Unconventional methods like ultrasonic-assisted extraction (UAE), microwave-assisted extraction (MAE), and supercritical fluid extraction (SFE) are highlighted for their efficiency and ability to reduce extraction times. The review concludes by comparing the advantages and disadvantages of various methodologies for drying, extraction, and separation of terpenes and terpenoids, emphasizing the need for optimization based on the specific characteristics of the compounds being extracted.This review discusses the factors influencing the extraction methods of terpenes and terpenoids from natural sources. Terpenes and terpenoids, secondary metabolites found in various organisms, are of industrial interest due to their diverse applications. The extraction process is influenced by several factors, including drying methods, which can affect the recovery and quality of terpenes. Soxhlet extraction is commonly used due to its high efficiency, but unconventional methods like microwave and high-pressure extraction, as well as unconventional solvents, have shown promising results. The review also covers the preparation of biological material, including the selection of appropriate tissue and the importance of fragmentation methods. Dry and wet fragmentation methods are discussed, with dry methods being more cost-effective but requiring careful moisture control. Wet methods preserve the quality of the final product and are useful for recovering high-polarity molecules. The choice of extraction methods, such as steam distillation, adsorption-based techniques, and organic solvents, is crucial for recovering volatile and non-volatile terpenoids. Unconventional methods like ultrasonic-assisted extraction (UAE), microwave-assisted extraction (MAE), and supercritical fluid extraction (SFE) are highlighted for their efficiency and ability to reduce extraction times. The review concludes by comparing the advantages and disadvantages of various methodologies for drying, extraction, and separation of terpenes and terpenoids, emphasizing the need for optimization based on the specific characteristics of the compounds being extracted.