The International Human Genome Sequencing Consortium (IHGSC) has completed the euchromatic sequence of the human genome, resulting in a highly accurate and nearly complete sequence with an error rate of approximately 1 event per 100,000 bases. The sequence contains 2.85 billion nucleotides and covers approximately 99% of the euchromatic genome, with only 341 gaps remaining. These gaps are primarily associated with segmental duplications and are challenging to resolve with current methods. The sequence is a significant improvement over the initial draft, which had many gaps and inaccuracies. The current sequence is the first near-complete sequence of a vertebrate genome and provides a solid foundation for biomedical research. The finishing process involved a complex iterative approach to resolve gaps and improve accuracy. The sequence was generated from 59,208 large-insert clones, with 45,742 clones sequenced to completion. The sequence was validated through various methods, including comparison with other genomes and analysis of cDNA and random genomic plasmids. The sequence is accurate and complete, with only a small percentage of the genome missing. The remaining gaps are primarily in heterochromatic regions and are challenging to resolve. The sequence has been annotated and deposited in public databases, and further research is needed to resolve the remaining gaps. The sequence provides a valuable resource for understanding the human genome and its role in biology and medicine.The International Human Genome Sequencing Consortium (IHGSC) has completed the euchromatic sequence of the human genome, resulting in a highly accurate and nearly complete sequence with an error rate of approximately 1 event per 100,000 bases. The sequence contains 2.85 billion nucleotides and covers approximately 99% of the euchromatic genome, with only 341 gaps remaining. These gaps are primarily associated with segmental duplications and are challenging to resolve with current methods. The sequence is a significant improvement over the initial draft, which had many gaps and inaccuracies. The current sequence is the first near-complete sequence of a vertebrate genome and provides a solid foundation for biomedical research. The finishing process involved a complex iterative approach to resolve gaps and improve accuracy. The sequence was generated from 59,208 large-insert clones, with 45,742 clones sequenced to completion. The sequence was validated through various methods, including comparison with other genomes and analysis of cDNA and random genomic plasmids. The sequence is accurate and complete, with only a small percentage of the genome missing. The remaining gaps are primarily in heterochromatic regions and are challenging to resolve. The sequence has been annotated and deposited in public databases, and further research is needed to resolve the remaining gaps. The sequence provides a valuable resource for understanding the human genome and its role in biology and medicine.