GEPIA2 is an enhanced web server for large-scale expression profiling and interactive analysis. Introduced in 2017, the original GEPIA web server provided valuable resources for gene expression analysis based on tumor and normal samples from TCGA and GTEx databases. GEPIA2 is an updated and enhanced version that offers higher resolution and more functionalities. It includes 198,619 isoforms and 84 cancer subtypes, allowing gene expression quantification at the transcript level and supporting analysis of specific cancer subtypes and comparisons between subtypes. GEPIA2 also incorporates new analysis techniques inspired by single-cell sequencing studies, enabling users to upload their own RNA-seq data and compare them with TCGA and GTEx samples. An API is provided for batch processing and easy retrieval of analysis results. The updated web server is publicly accessible at http://gepia2.cancer-pku.cn/. GEPIA2 provides features such as survival analysis, isoform usage profiling, and uploaded expression data comparison. It allows users to analyze existing data and upload their own data for analysis based on interactive functions. The server also provides a Python package for easy access from a command-line environment. New features include survival maps, isoform usage profiling, and cancer-subtype classification. Upgrades include isoform analysis, signature score analysis, and cancer subtype analysis. GEPIA2 facilitates the exploration of large TCGA and GTEx datasets for experimental scientists. It allows them to ask specific questions and test hypotheses at a higher resolution. GEPIA2 provides a direct toolset for addressing these needs. Based on the new features and improvements, users can focus on important tasks such as immune-cell clusters analysis and subtype-specific prognostic analysis. GEPIA2 is a preferred tool for experimental biologists and clinicians to explore big cancer genomics data.GEPIA2 is an enhanced web server for large-scale expression profiling and interactive analysis. Introduced in 2017, the original GEPIA web server provided valuable resources for gene expression analysis based on tumor and normal samples from TCGA and GTEx databases. GEPIA2 is an updated and enhanced version that offers higher resolution and more functionalities. It includes 198,619 isoforms and 84 cancer subtypes, allowing gene expression quantification at the transcript level and supporting analysis of specific cancer subtypes and comparisons between subtypes. GEPIA2 also incorporates new analysis techniques inspired by single-cell sequencing studies, enabling users to upload their own RNA-seq data and compare them with TCGA and GTEx samples. An API is provided for batch processing and easy retrieval of analysis results. The updated web server is publicly accessible at http://gepia2.cancer-pku.cn/. GEPIA2 provides features such as survival analysis, isoform usage profiling, and uploaded expression data comparison. It allows users to analyze existing data and upload their own data for analysis based on interactive functions. The server also provides a Python package for easy access from a command-line environment. New features include survival maps, isoform usage profiling, and cancer-subtype classification. Upgrades include isoform analysis, signature score analysis, and cancer subtype analysis. GEPIA2 facilitates the exploration of large TCGA and GTEx datasets for experimental scientists. It allows them to ask specific questions and test hypotheses at a higher resolution. GEPIA2 provides a direct toolset for addressing these needs. Based on the new features and improvements, users can focus on important tasks such as immune-cell clusters analysis and subtype-specific prognostic analysis. GEPIA2 is a preferred tool for experimental biologists and clinicians to explore big cancer genomics data.