GSDMD membrane pore formation is the mechanism of pyroptotic cell death. The study shows that GSDMD is essential for caspase-1-mediated cell death and cytokine release. GSDMD is cleaved by caspase-1, leading to membrane pore formation. The study demonstrates that GSDMD is targeted to the membrane and forms pores, leading to cell death. The study also shows that GSDMD is stable and monomeric in solution. The study also shows that GSDMD $ ^{1104N} $ is cleaved by caspase-1 and induces cell death. The study also shows that GSDMD $ ^{Nterm} $ forms pores in liposomes, as shown by cryo-EM. The study also shows that GSDMD does not form defined oligomeric structures when adsorbed to mica. The study also shows that the secondary structure of GSDMD is predicted by Jpred. The study provides evidence that GSDMD membrane pore formation is the mechanism of pyroptotic cell death.GSDMD membrane pore formation is the mechanism of pyroptotic cell death. The study shows that GSDMD is essential for caspase-1-mediated cell death and cytokine release. GSDMD is cleaved by caspase-1, leading to membrane pore formation. The study demonstrates that GSDMD is targeted to the membrane and forms pores, leading to cell death. The study also shows that GSDMD is stable and monomeric in solution. The study also shows that GSDMD $ ^{1104N} $ is cleaved by caspase-1 and induces cell death. The study also shows that GSDMD $ ^{Nterm} $ forms pores in liposomes, as shown by cryo-EM. The study also shows that GSDMD does not form defined oligomeric structures when adsorbed to mica. The study also shows that the secondary structure of GSDMD is predicted by Jpred. The study provides evidence that GSDMD membrane pore formation is the mechanism of pyroptotic cell death.