GSDS 2.0 is an upgraded gene feature visualization server that provides a more user-friendly interface, supports more types of annotation features and formats, and includes an integrated visual editor for editing generated figures. It also allows users to add a user-specified phylogenetic tree for evolutionary analysis. The server is freely available at http://gsds.cbi.pku.edu.cn and the full source code is also available for download. GSDS 2.0 supports multiple input formats including GenBank IDs, FASTA sequences, BED, GTF, and GFF3. Users can customize the size, shape, and color of annotation features, rescale introns, and edit generated figures interactively. The server also includes a dedicated panel for users to specify a phylogenetic tree for display alongside the main canvas. Compared to similar tools, GSDS 2.0 offers superior support for input/output formats, usability, and availability. The server has been widely used by scientists for functional and evolutionary studies of gene families. The authors have updated GSDS to version 2.0 to better serve the scientific community. The work was supported by several funding sources including the China National 973 Program and the State Key Laboratory of Protein and Plant Gene Research. The research of G.G. was supported in part by the National Outstanding Youth Talent Initiative Program. J.J. was supported partly by the China Postdoctoral Science Foundation Grant. No conflict of interest was declared.GSDS 2.0 is an upgraded gene feature visualization server that provides a more user-friendly interface, supports more types of annotation features and formats, and includes an integrated visual editor for editing generated figures. It also allows users to add a user-specified phylogenetic tree for evolutionary analysis. The server is freely available at http://gsds.cbi.pku.edu.cn and the full source code is also available for download. GSDS 2.0 supports multiple input formats including GenBank IDs, FASTA sequences, BED, GTF, and GFF3. Users can customize the size, shape, and color of annotation features, rescale introns, and edit generated figures interactively. The server also includes a dedicated panel for users to specify a phylogenetic tree for display alongside the main canvas. Compared to similar tools, GSDS 2.0 offers superior support for input/output formats, usability, and availability. The server has been widely used by scientists for functional and evolutionary studies of gene families. The authors have updated GSDS to version 2.0 to better serve the scientific community. The work was supported by several funding sources including the China National 973 Program and the State Key Laboratory of Protein and Plant Gene Research. The research of G.G. was supported in part by the National Outstanding Youth Talent Initiative Program. J.J. was supported partly by the China Postdoctoral Science Foundation Grant. No conflict of interest was declared.